| 货号 | 9539C |
| 描述 | The PathScan® Total Oct-4A Sandwich ELISA Kit is a solid phase sandwich enzyme-linked immunosorbent assay (ELISA) that detects endogenous levels of Oct-4A. An Oct-4A Rabbit Antibody has been coated onto the microwells. After incubation with cell lysates, Oct-4A protein is captured by the coated antibody. Following extensive washing, an Oct-4A Mouse Detection Antibody is added to detect the captured Oct-4A protein. Anti-mouse IgG, HRP-linked Antibody is then used to recognize the bound detection antibody. HRP substrate, TMB, is added to develop color. The magnitude of the absorbance for the developed color is proportional to the quantity of Oct-4A. Antibodies in kit are custom formulations specific to kit. |
| 反应种属 | Human |
| 应用 | ELISA |
| 目标/特异性 | PathScan® Total Oct-4A Sandwich ELISA Kit detects endogenous levels of total Oct-4A protein. This kit detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species. |
| 供应商 | CST |
| 背景 | Oct-4 (POU5F1) is a transcription factor highly expressed in undifferentiated embryonic stem cells and embryonic germ cells (1). A network of key factors that includes Oct-4, Nanog, and Sox2 is necessary for the maintenance of pluripotent potential, and downregulation of Oct-4 has been shown to trigger cell differentiation (2,3). Research studies have demonstrated that Oct-4 is a useful germ cell tumor marker (4). Oct-4 exists as two splice variants, Oct-4A and Oct-4B (5). Recent studies have suggested that the Oct-4A isoform has the ability to confer and sustain pluripotency, while Oct-4B may exist in some somatic, non-pluripotent cells (6,7). |
| 存放说明 | 4C |
| 参考文献 | Looijenga, L.H. et al. (2003) Cancer Res 63, 2244-50. Pesce, M. and Schöler, H.R. (2001) Stem Cells 19, 271-278. Pan, G. and Thomson, J.A. (2007) Cell Res 17, 42-9. Cheng, L. et al. (2007) J Pathol 211, 1-9. Takeda, J. et al. (1992) Nucleic Acids Res 20, 4613-20. Cauffman, G. et al. (2006) Stem Cells 24, 2685-91. Lee, J. et al. (2006) J Biol Chem 281, 33554-65. |
Figure 2. The relationship between the protein concentration of lysates from HeLa and NTERA-2 cl.D1 cells and the absorbance at 450 nm is shown. Cells were lysed at 90% confluency. 图2. 如图所示，HeLa细胞和NTERA-2 cl.D1细胞裂解物蛋白浓度与450nm处吸光度值的关系。细胞长至90%汇合时进行裂解。 | |
Figure 1. The expression of Oct-4A can only be detected in embryonic cells, such as NTERA-2 cl.D1 cells, but not in non-embryonic cells, such as HeLa cells, as detected by the PathScan® Total Oct-4A Sandwich ELISA Kit. HeLa and NTERA-2 cl.D1 cells (90% confluent) were lysed. The absorbance readings at 450 nm are shown in the top figure, while the corresponding western blot using Oct-4A (C30A3) Rabbit mAb #2840 is shown in the bottom figure. 图1. 用PathScan® Total Oct-4A Sandwich ELISA Kit只能在胚胎细胞如NTERA-2 cl.D1细胞中检测到Oct-4A，而不能在非胚胎细胞如HeLa细胞中检测到。HeLa细胞和NTERA-2 cl.D1细胞（90%汇合）裂解。上图显示450nm处的吸光度值，而相应的western blot结果见下图，western blot实验使用的抗体为Oct-4A (C30A3) Rabbit mAb #2840. |
