| 货号 | 13127C |
| 反应种属 | Human |
| 应用 | ELISA |
| 目标/特异性 | PathScan® HIF-1α Sandwich ELISA Kit recognizes endogenous levels of HIF-1α protein in human cells, as shown in Figure 1. The kit sensitivity is shown in Figure 2. This kit detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species. |
| 供应商 | CST |
| 背景 | Hypoxia-inducible factor 1 (HIF1) is a heterodimeric transcription factor that plays a critical role in the cellular response to hypoxia (1). The HIF1 complex consists of two subunits, HIF-1α and HIF-1β, which are basic helix-loop-helix proteins of the PAS (Per, ARNT, Sim) family (2). HIF1 regulates the transcription of a broad range of genes that facilitate responses to the hypoxic environment, including genes regulating angiogenesis, erythropoiesis, cell cycle, metabolism, and apoptosis. The widely expressed HIF-1α is typically degraded rapidly in normoxic cells by the ubiquitin/proteasomal pathway. Under normoxic conditions, HIF-1α is proline hydroxylated leading to a conformational change that promotes binding to the von Hippel Lindau protein (VLH) E3 ligase complex; ubiquitination and proteasomal degradation follows (3,4). Both hypoxic conditions and chemical hydroxylase inhibitors (such as desferrioxamine and cobalt) inhibit HIF-1α degradation and lead to its stabilization. In addition, HIF-1α can be induced in an oxygen-independent manner by various cytokines through the PI3K-AKT-mTOR pathway (5-7). |
| 存放说明 | 4C |
| 参考文献 | Sharp, F.R. and Bernaudin, M. (2004) Nat Rev Neurosci 5, 437-48. Wang, G.L. et al. (1995) Proc Natl Acad Sci U S A 92, 5510-4. Jaakkola, P. et al. (2001) Science 292, 468-72. Maxwell, P.H. et al. (1999) Nature 399, 271-5. Fukuda, R. et al. (2002) J Biol Chem 277, 38205-11. Jiang, B.H. et al. (2001) Cell Growth Differ 12, 363-9. Laughner, E. et al. (2001) Mol Cell Biol 21, 3995-4004. Walisser, J.A. et al. (2004) Proc Natl Acad Sci U S A 101, 16677-82. Salomon-Nguyen, F. et al. (2000) Proc Natl Acad Sci U S A 97, 6757-62. Gunton, J.E. et al. (2005) Cell 122, 337-49. |
