| 货号 | 80251C |
| 反应种属 | Human |
| 应用 | ELISA |
| 目标/特异性 | PathScan® Total Estrogen Receptor α Sandwich ELISA Kit detects endogenous levels of estrogen receptor α protein in human cells, as shown in Figure 1. The kit sensitivity is shown in Figure 2. This kit detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species. |
| 供应商 | CST |
| 背景 | Estrogen receptor α (ERα), a member of the steroid receptor superfamily, contains highly conserved DNA binding and ligand binding domains (1). Through its estrogen-independent and estrogen-dependent activation domains (AF-1 and AF-2, respectively), ERα regulates transcription by recruiting coactivator proteins and interacting with general transcriptional machinery (2). Phosphorylation at multiple sites provides an important mechanism to regulate ERα activity (3-5). Ser104, 106, 118, and 167 are located in the amino-terminal transcription activation function domain AF-1, and phosphorylation of these serine residues plays an important role in regulating ERα activity. Ser118 may be the substrate of the transcription regulatory kinase CDK7 (5). Ser167 may be phosphorylated by p90RSK and Akt (4,6). According to the research literature, phosphorylation at Ser167 may confer tamoxifen resistance in breast cancer patients (4). |
| 存放说明 | 4C |
| 参考文献 | Mangelsdorf, D.J. et al. (1995) Cell 83, 835-9. Glass, C.K. and Rosenfeld, M.G. (2000) Genes Dev 14, 121-41. Chen, D. et al. (1999) Mol Cell Biol 19, 1002-15. Campbell, R.A. et al. (2001) J Biol Chem 276, 9817-24. Chen, D. et al. (2000) Mol Cell 6, 127-37. Joel, P.B. et al. (1998) Mol Cell Biol 18, 1978-84. Burns, K.A. and Korach, K.S. (2012) Arch Toxicol 86, 1491-504. |
Figure 1. Estrogen receptor α protein is detected from multiple cell lines using the PathScan® Total Estrogen Receptor α Sandwich ELISA Kit #80251. The absorbance readings at 450 nm are shown in the top figure, while the corresponding western blot using Estrogen Receptor α (D6R2W) Rabbit mAb #13258 is shown in the bottom figure. | |
Figure 2. The relationship between protein concentration of lysates from MCF7 cells and the absorbance at 450 nm as detected by the PathScan® Total Estrogen Receptor α Sandwich ELISA Kit #80251 is shown. MCF7 cells (85% confluence) were harvested and then lysed. |
