| 货号 | 7291C |
| 描述 | The PathScan® Total Cox2 Sandwich ELISA Kit is a solid phase sandwich enzyme-linked immunosorbent assay (ELISA) that detects endogenous levels of Cox2. A Cox2 Mouse Antibody has been coated onto the microwells. After incubation with cell lysates, Cox2 protein is captured by the coated antibody. Following extensive washing, a Cox2 Rabbit Detection Antibody is added to detect the captured Cox2 protein. Anti-rabbit IgG, HRP-linked Antibody is then used to recognize the bound detection antibody. HRP substrate, TMB, is added to develop color. The magnitude of the absorbance for the developed color is proportional to the quantity of Cox2. Antibodies in kit are custom formulations specific to kit.CST PathScan® Total Cox2 Sandwich ELISA Kit是固相夹心酶联免疫吸收剂检测试剂盒(ELISA)，它能够检测内源水平Cox2蛋白。 Cox2鼠抗包裹到微孔的外表面。然后与细胞裂解液孵育，Cox2蛋白能够被抗体捕获。经过严格的冲洗后，Cox2兔检测抗体加入检测捕捉到的Cox2蛋白。抗兔IgG, HRP连接抗体用于识别检测抗体的结合。HRP的底物TMB加入进行颜色反应。对反应的颜色，光密度的数值按比例换算成Cox2蛋白的量。试剂盒中的抗体是经过客户证实试剂盒特异的。 |
| 反应种属 | Human |
| 应用 | ELISA |
| 目标/特异性 | PathScan® Total Cox2 Sandwich ELISA Kit detects endogenous levels of total Cox2 protein. This kit detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species. |
Figure 1. Treatment of CCD-1070Sk cells with IL-1β stimulates expression of Cox2 as detected by the PathScan® Total Cox2 Sandwich ELISA Kit. CCD-1070Sk cells (80-90% confluent) were serum starved or treated with hIL-1β #8900 (5 ng/ml, 16 hr). The absorbance readings at 450 nm are shown in the top figure, while the corresponding western blot using Cox2 Antibody #4842 is shown in the bottom figure.图1.用PathScan® Total Cox2 Sandwich ELISA Kit检测经过IL-1β刺激表达 Cox2的 CCD-1070Sk细胞。CCD-1070Sk 细胞(80-90% confluent) 经过血清饥饿或经过hIL-1β #8900 (5 ng/ml, 16 hr)处理，OD 450 的读数在图片的最上面显示, 同时对应的Western免疫印迹在下面图片显示，所用抗体为Cox2 Antibody #4842。 | |
Figure 2. The relationship between the protein concentration of lysates from CCD-1070Sk cells, serum starved or hIL-1β-treated, and the absorbance at 450 nm is shown. CCD-1070Sk cells (80-90% confluent) were serum starved or treated with hIL-1β #8900 (5 ng/ml, 16 hr) and then lysed.图2.图示为经过血清饥饿或hIL-1β处理的CCD-1070Sk细胞裂解液蛋白浓度与试剂盒实验OD450读数之间的关系。CCD-1070Sk 细胞(80-90% confluent)经过血清饥饿或经过hIL-1β #8900 (5 ng/ml, 16 hr)处理然后裂解。 |
