| 货号 | 12321C |
| 描述 | The PathScan® Phospho-Ret (panTyr) Chemiluminescent Sandwich ELISA Kit is a solid phase sandwich enzyme-linked immunosorbent assay (ELISA) that detects endogenous levels of tyrosine-phosphorylated Ret protein with a chemiluminescent readout. Chemiluminescent ELISAs often have a wider dynamic range and higher sensitivity than conventional chromogenic detection. This chemiluminescent ELISA, which is offered in low volume microplates, shows increased signal and sensitivity while using a smaller sample size. A Ret Rabbit mAb has been coated onto the microwells. After incubation with cell lysates, Ret protein (phospho and nonphospho) is captured by the coated antibody. Following extensive washing, a Phospho-Tyrosine Mouse Detection mAb is added to detect captured tyrosine-phosphorylated Ret protein. Anti-mouse IgG, HRP-linked Antibody is then used to recognize the bound detection antibody. Chemiluminescent reagent is added for signal development. The magnitude of light emission, measured in relative light units (RLU), is proportional to the quantity of tyrosine-phosphorylated Ret protein. Antibodies in kit are custom formulations specific to kit. |
| 反应种属 | Human |
| 应用 | ELISA |
| 目标/特异性 | PathScan® Phospho-Ret (panTyr) Chemiluminescent Sandwich ELISA Kit #12321 detects endogenous levels of tyrosine-phosphorylated Ret protein in human cells. This kit detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species. |
| 供应商 | CST |
| 背景 | The Ret proto-oncogene (c-Ret) is a receptor tyrosine kinase that functions as a multicompetent receptor complex in conjunction with other membrane-bound, ligand-binding GDNF family receptors (1). Ligands that bind the Ret receptor include the glial cell line-derived neurotropic factor (GDNF) and its congeners neurturin, persephin, and artemin (2-4). Research studies have shown that alterations in the corresponding RET gene are associated with diseases including papillary thyroid carcinoma, multiple endocrine neoplasia (type 2A and 2B), familial medullary thyroid carcinoma, and a congenital developmental disorder known as Hirschsprung’s disease (1,3). The Tyr905 residue located in the Ret kinase domain plays a crucial role in Ret catalytic and biological activity. Substitution of Phe for Tyr at position 905 dramatically inhibits Ret autophosphorylation activity (5). |
| 存放说明 | 4C |
| 参考文献 | Airaksinen, M.S. et al. (1999) Mol Cell Neurosci 13, 313-25. Takahashi, M. et al. (1989) Oncogene 4, 805-6. Manié, S. et al. (2001) Trends Genet 17, 580-9. Tallini, G. and Asa, S.L. (2001) Adv Anat Pathol 8, 345-54. Iwashita, T. et al. (1999) Oncogene 18, 3919-22. |
Figure 1. The relationship between protein concentration of lysates, untreated (Nonphospho) and phosphatase inhibitor treated (Phospho), and immediate light generation with chemiluminescent substrate is shown. TT cells were cultured (85% confluent) and lysed with the addition of phosphatase inhibitor to the PathScan® Sandwich ELISA Cell Lysis Buffer (1X) #9803. Graph inset corresponding to the shaded area shows high sensitivity and a linear response at the low protein concentration range.图1.如图所示裂解物蛋白浓度之间的关系，细胞不处理（非磷酸化）或用磷酸酶抑制剂处理（磷酸化），立即产生光化学发光底物。TT细胞培养（85%汇合）后，加入磷酸酶抑制剂，用PathScan® Sandwich ELISA Cell Lysis Buffer (1X) #9803进行裂解。图中对应的阴影部分的面积图形显示了高灵敏度和低蛋白质浓度范围内的线性响应。 |
