| 货号 | 13340C |
| 反应种属 | Human |
| 应用 | ELISA |
| 目标/特异性 | PathScan® Phospho-Mer (panTyr) Sandwich ELISA Kit recognizes endogenous levels of tyrosine-phosphorylated Mer protein in human cells, as shown in Figure 1. The kit sensitivity is shown in Figure 2. This kit detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species. |
Figure 2. The relationship between protein concentration of phospho or nonphospho lysates and the absorbance at 450 nm as detected by the PathScan® Phospho-Mer (panTyr) Sandwich ELISA Kit is shown. Unstarved HCC827 cells were cultured (85% confluence) and lysed with or without addition of phosphatase inhibitors to the lysis buffer (phospho or nonphospho lysate, respectively). | |
Figure 1. Constitutive phosphorylation of Mer in HCC827 cells lysed in the presence of phosphatase inhibitors (phospho lysate) is detected by PathScan® Phospho-Mer (panTyr) Sandwich ELISA Kit #13340. In contrast, a low level of phospho-Mer protein is detected in HCC827 cells lysed in the absence of phosphatase inhibitors (nonphospho lysate). Similar levels of total Mer protein from both nonphospho and phospho lysates are detected by PathScan® Total Mer Sandwich ELISA Kit #13488 (upper, left). Absorbance at 450 nm is shown in the top figure while corresponding western blots using Mer (D21F11) XP® Rabbit mAb #4319 (left) and a phospho-Mer (Tyr749/753) antibody (right) are shown in the bottom figure. Phosphatase inhibitors include sodium pyrophosphate, β-glycerophosphate, and sodium orthovanadate. |
