| 货号 | 7941C |
| 描述 | The PathScan® Phospho-Lck (Tyr505) Sandwich ELISA Kit is a solid phase sandwich enzyme-linked immunosorbent assay (ELISA) that detects endogenous levels of phospho-Lck (Tyr505). A phospho-Lck rabbit antibody has been coated onto the microwells. After incubation with cell lysates, phospho-Lck (Tyr505) is captured by the coated antibody. Following extensive washing, a Lck mouse detection mAb is added to detect the captured phospho-Lck (Tyr505). Anti-mouse, HRP-linked antibody is then used to recognize the bound detection antibody. The HRP substrate TMB is added to develop color. The magnitude of the absorbance for this developed color is proportional to the quantity of phospho-Lck (Tyr505). |
| 反应种属 | Human |
| 应用 | ELISA |
| 目标/特异性 | The PathScan® Phospho-Lck (Tyr505) Sandwich ELISA Kit from Cell Signaling Technology detects endogenous levels of Lck when phosphorylated at Tyr505, as shown in Figure 1. This kit does not cross-react with the Src family members Yes, Src, Lyn or Hck. Other family members have not been tested. The kit sensitivity is shown in Figure 2. This kit detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species. |
| 供应商 | CST |
| 背景 | Lck belongs to the Src-like non-receptor tyrosine kinase family with the typical Src family kinase structure: a unique amino terminal domain (Src homology 4 domain, SH4) followed by an SH3 domain, an SH2 domain, a kinase domain (SH1), and a carboxy-terminal negative regulatory domain (1). Lck activity is controlled by the interactions of SH2 and SH3 domains as well as tyrosine phosphorylation status of the activation loop (2,3). Lck is recruited to the T cell receptor (TCR) complex upon stimulation and activates downstream tyrosine kinases to initiate T cell signaling (4). Lck is also found to be involved in the regulation of mitochondrial apoptosis pathways and may be responsible for some anticancer drug induced apoptosis (5,6). |
| 存放说明 | 4C |
| 参考文献 | Palacios, E.H. and Weiss, A. (2004) Oncogene 23, 7990-8000. Mustelin, T. and Taskén, K. (2003) Biochem J 371, 15-27. Gervais, F.G. et al. (1993) Mol Cell Biol 13, 7112-21. Straus, D.B. and Weiss, A. (1992) Cell 70, 585-93. Belka, C. et al. (2003) Oncogene 22, 176-85. Gruber, C. et al. (2004) Biochem Pharmacol 67, 1859-72. |
Figure 1. Treatment of Jurkat cells with H202 stimulates phosphorylation of Lck at Tyr505 as detected by the PathScan® Phospho-Lck (Tyr505) Sandwich ELISA Kit #7941, but does not affect the level of total Lck. Jurkat cells were treated with 20 mM H202 for 3 minutes at 37ºC. In untreated lysates, λ-phosphatase was used to abolish basal phosphorylation. The absorbance readings at 450 nm are shown in the top figure, while the western blots, using Lck (L22B1) Mouse mAb #2657 (lower panel) or Phospho-Lck (Tyr505) Antibody #2751 (upper panel), are shown in the bottom figure.图1.PathScan® Phospho-Lck (Tyr505) Sandwich ELISA Kit #7941检测Jurkat细胞中经H202处理诱导Tyr505位点磷酸化Lck蛋白，但是不影响总Lck蛋白。在37oC条件下，Jurkat细胞经过20 mM H202处理3min。在未经处理的裂解液中，用λ-phosphatase对本底的磷酸化进行消除。OD450的读数在图片的最上面显示,同时对应的Western免疫印迹在下面图片显示，所用抗体为Lck (L22B1) Mouse mAb #2657(下侧图) 或Phospho-Lck (Tyr505) Antibody #2751(上侧图)。 | |
Figure 2. The relationship between lysate protein concentration from untreated and H202-treated Jurkat cells and the absorbance at 450 nm using PathScan® Phospho-Lck (Tyr505) Sandwich ELISA Kit #7941 is shown. Jurkat cells were treated with H202 (20 mM) for 3 minutes at 37ºC and then lysed.图2.用 PathScan® Phospho-Lck (Tyr505) Sandwich ELISA Kit #7941验证未经处理和经H202处理的Jurkat细胞的裂解液蛋白浓度与450 nm吸光度之间的关系。在37oC条件下，Jurkat细胞经过20 mM H202处理3min随后裂解。 |
