| 货号 | 7986C |
| 描述 | The PathScan® Phospho-Acetyl-CoA Carboxylase (Ser79) Sandwich ELISA Kit is a solid phase sandwich enzyme-linked immunosorbent assay (ELISA) that detects endogenous levels of acetyl-CoA carboxylase (ACC) when phosphorylated at Ser79. A phospho-ACC (Ser79) rabbit antibody has been coated onto the microwells. After incubation with cell lysates, phospho-ACC protein is captured by the coated antibody. Following extensive washing, an ACC mouse detection mAb is added to detect the captured ACC protein. Anti-mouse IgG, HRP-linked antibody is then used to recognize the bound detection antibody. HRP substrate, TMB, is added to develop color. The magnitude of the absorbance for the developed color is proportional to the quantity of ACC phosphorylated at Ser79. Antibodies in kit are custom formulations specific to kit. |
| 反应种属 | Human |
| 应用 | ELISA |
| 目标/特异性 | PathScan® Phospho-Acetyl-CoA Carboxylase (Ser79) Sandwich ELISA Kit detects endogenous levels of ACC protein when phosphorylated at Ser79 as shown in Figure 1. Kit sensitivity is shown in Figure 2. This kit detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species. |
| 供应商 | CST |
| 背景 | Acetyl-CoA carboxylase (ACC) catalyzes the pivotal step of the fatty acid synthesis pathway. The 265 kDa ACCα (ACC1) is the predominant isoform found in liver, adipocytes, and mammary gland, while the 280 kDa ACCβ (ACC2) is the major isoform in skeletal muscle and heart (1). Phosphorylation by AMPK at Ser79 or by PKA at Ser1200 inhibits the enzymatic activity of ACC (2). ACC is a potential target of anti-obesity drugs (3,4). |
| 存放说明 | 4C |
| 参考文献 | Castle, J.C. et al. (2009) PLoS One 4, e4369. Kreuz, S. et al. (2009) Diabetes Metab Res Rev 25, 577-86. Ha, J. et al. (1994) J Biol Chem 269, 22162-8. Abu-Elheiga, L. et al. (2001) Science 291, 2613-6. Levert, K.L. et al. (2002) J Biol Chem 277, 16347-50. |
Figure 2. The relationship between the protein concentration of lysates from untreated and H2O2-treated Hep G2 cells and the absorbance at 450 nm using the PathScan® Phospho-Acetyl-CoA Carboxylase (Ser79) Sandwich ELISA Kit is shown. 图2，细胞裂解液蛋白浓度与450钠米吸光度的关系图，细胞未处理或用过氧化氢处理，所用试剂盒为PathScan® Phospho-Acetyl-CoA Carboxylase (Ser79) Sandwich ELISA Kit 。 | |
Figure 1. Treatment of Hep G2 cells with H2O2 stimulates phosphorylation of ACC at Ser79, detected by the PathScan® Phospho-ACC (Ser79) Sandwich ELISA Kit #7986, but does not affect the levels of total ACC detected by PathScan® Total ACC Sandwich ELISA Kit #7996. Hep G2 cells (80-90% confluent) were treated 10 mM hydrogen peroxide for 10 minutes and lysed with #7018. The absorbance readings at 450 nm are shown in the top figure, while the corresponding western blots using Acetyl-CoA Carboxylase (C83B10) Rabbit mAb #3676 (left panel) or Phospho-Acetyl-CoA Carboxylase (Ser79) Antibody #3661 (right panel) are shown in the bottom figure. 图1，用过氧化氢处理Hep G2细胞会增加ACC在Ser79位点的磷酸化水平，检测试剂盒为PathScan? Phospho-ACC (Ser79) Sandwich ELISA Kit #7986。但不影响ACC总蛋白水平，检测试剂盒为PathScan? Total ACC Sandwich ELISA Kit #7996。Hep G2用10 mM过氧化氢处理10分钟，然后用#7018裂解。上图所示为450nm处的吸光度值，下图所示为相应的Western blot检测，所用抗体为Acetyl-CoA Carboxylase (C83B10) Rabbit mAb兔单抗 #3676(左)，Phospho-Acetyl-CoA Carboxylase (Ser79) Antibody #3661(右)。 |
