| 货号 | 7194C |
| 描述 | PathScan® Cleaved Notch1 (Val1744) Sandwich ELISA Kit from Cell Signaling Technology is a solid phase sandwich enzyme-linked immunosorbent assay (ELISA) that detects endogenous levels of Notch1 when cleaved at Val1744. A Notch1 rat antibody has been coated onto the microwells. After incubation with cell lysates, Notch1 protein is captured by the coated antibody. Following extensive washing, a cleaved Notch1 (Val1744) Rabbit detection antibody is added to detect the captured Notch1 protein. Anti-Rabbit IgG, HRP-linked Antibody is then used to recognize the bound detection antibody. HRP substrate, TMB, is added to develop color. The magnitude of the absorbance for this developed color is proportional to the quantity of cleaved Notch1 (Val1744). |
| 反应种属 | Human |
| 应用 | ELISA |
| 目标/特异性 | PathScan® Cleaved Notch1 (Val1744) Sandwich ELISA Kit #7194 detects endogenous levels of Notch1 protein when cleaved at Val1744 as shown in Figure 1. The kit sensitivity is shown in Figure 2. This kit detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species. |
| 供应商 | CST |
| 背景 | Notch1 is a transmembrane protein functioning in development and the determination of cell fate (1). During maturation, the notch molecule is cleaved by a furin-like convertase at its extracellular domain (2). Upon binding to a ligand such as Delta1, or upon extracellular calcium depletion, the carboxy-terminal notch1 fragment is released and further cleaved between Gly1743 and Val1744 (3,4). The resulting activated cytosolic fragment translocates to the nucleus where it activates transcription. |
| 存放说明 | 4C and -20C |
| 参考文献 | Artavanis-Tsakonas, S. et al. (1999) Science 284, 770-6. Chan, Y.M. and Jan, Y.N. (1998) Cell 94, 423-6. Schroeter, E.H. et al. (1998) Nature 393, 382-6. Rand, M.D. et al. (2000) Mol Cell Biol 20, 1825-35. |
Figure 2. The relationship between the protein concentration of lysates from Jurkat cells, untreated or treated with γ secretase inhibitor, and the absorbance at 450 nm using the PathScan® Cleaved Notch1 (Val1744) Sandwich ELISA Kit #7194 is shown. | |
图2，使用 PathScan® Cleaved Notch1 (Val1744) Sandwich ELISA Kit #7194 检测未处理或经过 γ 分泌酶抑制剂处理的Jurkat细胞裂解物，其蛋白浓度与450 nm处吸光值间的关系如图所示。 |
