PathScan® Phospho-eNOS (Ser1177) Sandwich ELISA Kit【科瑞奥博】

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PathScan® Phospho-eNOS (Ser1177) Sandwich ELISA Kit

货号： 7980C 基本售价： 6346.0 元规格： 1Kit

产品信息

概述
货号	7980C
描述	The PathScan® Phospho-eNOS (Ser1177) Sandwich ELISA Kit is a solid phase sandwich enzyme-linked immunosorbent assay (ELISA) that detects endogenous levels of eNOS when phosphorylated at Ser1177. A phospho-eNOS (Ser1177) rabbit monoclonal antibody has been coated onto the microwells. After incubation with cell lysates, phospho-eNOS protein is captured by the coated antibody. Following extensive washing, an eNOS mouse monoclonal detection antibody is added to detect captured eNOS protein phosphorylated at Ser1177. HRP-linked streptavidin is then used to recognize the bound detection antibody. HRP substrate (TMB) is added to develop color. The magnitude of the absorbance for this developed color is proportional to the quantity of eNOS phosphorylated at Ser1177.
反应种属	Bovine
应用	ELISA
目标/特异性	PathScan® Phospho-eNOS (Ser1177) Sandwich ELISA Kit #7980 detects endogenous levels of eNOS protein only when phosphorylated at Ser1177 (see Figure 1). The kit sensitivity is shown in Figure 2. This kit detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species.

性能
供应商	CST
背景	Endothelial nitric-oxide synthase (eNOS) is an important enzyme in the cardiovascular system. It catalyzes the production of nitric oxide (NO), a key regulator of blood pressure, vascular remodeling, and angiogenesis (1,2). The activity of eNOS is regulated by phosphorylation at multiple sites. The two most thoroughly studied sites are the activation site Ser1177 and the inhibitory site Thr495 (3). Several protein kinases including Akt/PKB, PKA, and AMPK activate eNOS by phosphorylating Ser1177 in response to various stimuli (4,5). In contrast, bradykinin and H2O2 activate eNOS activity by promoting both Ser1177 phosphorylation and Thr495 dephosphorylation (6,7).
存放说明	4C
参考文献	Fulton, D. et al. (2001) J Pharmacol Exp Ther 299, 818-24. Shaul, P.W. (2002) Annu Rev Physiol 64, 749-74. Chen, Z.P. et al. (1999) FEBS Lett 443, 285-9. Dimmeler, S. et al. (1999) Nature 399, 601-5. Fulton, D. et al. (1999) Nature 399, 597-601. Harris, M.B. et al. (2001) J Biol Chem 276, 16587-91. Thomas, S.R. et al. (2002) J Biol Chem 277, 6017-24.

参考图片

Figure 1. Treatment of BAEC cells with 500 µM of H2O2 increases phosphorylation of eNOS at Ser1177, detected by the PathScan® Phospho-eNOS (Ser1177) Sandwich ELISA Kit #7980. The absorbance readings at 450 nm are shown in the top figure, while the corresponding western blots using eNOS Antibody #9572 (left panel) or Phospho-eNOS (Ser1177) (C9C3) Rabbit mAb #9570 (right panel) are shown in the bottom figure.

图1，采用500 uM的过氧化氢处理BAEC细胞，使得eNOS蛋白在Ser1177位点发生磷酸化，检测用试剂盒为PathScan ® Phospho-eNOS (Ser1177) Sandwich ELISA Kit #7980 。在450nm处读取吸光度值（上图），相对应western blot采用抗体为eNOS Antibody #9572 (左泳道)或Phospho-eNOS (Ser1177) (C9C3) Rabbit mAb兔单抗 #9570 (右泳道），见下图。