| 货号 | 39412C |
| 反应种属 | Human |
| 目标/特异性 | PathScan® Total Tyro3 Sandwich ELISA Kit recognizes endogenous levels of Tyro3 protein in human cells, as shown in Figure 1. The kit sensitivity is shown in Figure 2. This kit detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species. |
| 使用方法 | ELISA |
| 供应商 | CST |
| 背景 | Tyro3 is a receptor tyrosine kinase belonging to the TAM subfamily (Tyro3, Axl and Mer). All three members have similar domain structure composed of an extracellular region with 2 Ig-like domains, followed by 2 FNII-like domains, a single transmembrane region, and a cytoplasmic tyrosine kinase domain (1). The natural ligand for Tyro3, as well as Axl and Mer, is Gas6 (growth arrest-specific gene 6) (1,2). Expression pattern and target knockout data indicate an important role of Tyro3 in apoptotic cell phagocytosis of dendritic cells and macrophages (3), NK cell differentiation (4), reproductive neuron survival and migration (5), osteoclast stimulation (6,7), as well as cortical and hippocampal neuron function (8). Both MAPK and PI3K pathways have been suggested as downstream targets of Tyro3 activation (7,8). Tyro3 has also been shown to be correlated to melanoma tumorigenesis, likely through its reglulatory role in the expression of oncogenic microphthalmia-associated transcription factor (MITF) (9). |
| 存放说明 | 4C |
| 参考文献 | Binder, M.D. and Kilpatrick, T.J. (2009) Neurosignals 17, 277-87. Nagata, K. et al. (1996) J Biol Chem 271, 30022-7. Seitz, H.M. et al. (2007) J Immunol 178, 5635-42. Caraux, A. et al. (2006) Nat Immunol 7, 747-54. Pierce, A. et al. (2008) Mol Endocrinol 22, 2481-95. Nakamura, Y.S. et al. (1998) Stem Cells 16, 229-38. Katagiri, M. et al. (2001) J Biol Chem 276, 7376-82. Prieto, A.L. et al. (2007) Neuroscience 150, 319-34. Zhu, S. et al. (2009) Proc Natl Acad Sci U S A 106, 17025-30. |
Figure 1. Constitutive phosphorylation of Tyro3 in CHO/Tyro3 cells lysed in the presence of phosphatase inhibitors (phospho lysate) is detected by PathScan® Phospho-Tyro3 (panTyr) Sandwich ELISA Kit #53402. In contrast, a low level of phospho-Tyro3 protein is detected in CHO/Tyro3 cells lysed in the absence of phosphatase inhibitors (nonphospho lysate). Similar levels of total Tyro3 protein from both nonphospho and phospho lysates are detected by PathScan® Total Tyro3 Sandwich ELISA Kit (upper, left). Absorbance at 450 nm is shown in the top figure while corresponding western blots using Tyro3 (D38C6) Rabbit mAb #5585 (left) and a Phospho-Tyrosine Mouse mAb #9411 (right) are shown in the bottom figure. Phosphatase inhibitors include sodium pyrophosphate, β-glycerophosphate, and sodium orthovanadate. | |
CHO/Tyro3 cells are derived from CHO cells tranfected with human Tyro3. |
