| 货号 | 53553C |
| 反应种属 | Human |
| 目标/特异性 | PathScan® Total MUC1 Sandwich ELISA Kit detects endogenous levels of MUC1 protein in human cells, as shown in Figure 1. The kit sensitivity is shown in Figure 2. This kit detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species. |
| 使用方法 | ELISA |
| 供应商 | CST |
| 背景 | Mucins represent a family of glycoproteins characterized by repeat domains and dense O-glycosylation (1). MUC1 (or mucin 1) is aberrantly overexpressed in most human carcinomas. Increased expression of MUC1 in carcinomas reduces cell-cell and cell-ECM interactions. MUC1 is cleaved proteolytically, and the large ectodomain can remain associated with the small 25 kDa carboxy-terminal domain that contains a transmembrane segment and a 72-residue cytoplasmic tail (1). MUC1 interacts with ErbB family receptors and potentiates ERK1/2 activation (2). MUC1 also interacts with β-catenin, which is regulated by GSK-3β, PKCγ, and Src through phosphorylation at Ser44, Thr41, and Tyr46 of the MUC1 cytoplasmic tail (3-5). Overexpression of MUC1 potentiates transformation (6) and attenuates stress-induced apoptosis through the Akt or p53 pathways (7,8). |
| 存放说明 | 4C |
| 参考文献 | Baldus, S.E. et al. (2004) Crit Rev Clin Lab Sci 41, 189-231. Schroeder, J.A. et al. (2001) J Biol Chem 276, 13057-64. Li, Y. et al. (1998) Mol Cell Biol 18, 7216-24. Li, Y. et al. (2001) J Biol Chem 276, 6061-4. Ren, J. et al. (2002) J Biol Chem 277, 17616-22. Schroeder, J.A. et al. (2004) Oncogene 23, 5739-47. Raina, D. et al. (2004) J Biol Chem 279, 20607-12. Wei, X. et al. (2005) Cancer Cell 7, 167-78. |
Figure 1. Detection of MUC1 protein in ZR-75-1, MCF7, and HEK293 cells is examined by using the PathScan® Total MUC1 Sandwich ELISA Kit. The absorbance readings at 450 nm are shown in the top figure, while the corresponding Western blot using MUC1 (D9O8K) Rabbit mAb #14161 is shown in the bottom figure. | |
Figure 2. The relationship between protein concentration of lysates from ZR-75-1 cels and the absorbance at 450 nm as detected by the PathScan® Total MUC1 Sandwich ELISA Kit is shown. Unstarved ZR-75-1 cells (85% confluence) were harvested and then lysed. |
