| 货号 | 53804C |
| 反应种属 | Human\Mouse\Moneky |
| 目标/特异性 | PathScan® Total HDAC4 Sandwich ELISA Kit detects endogenous levels of HDAC4 protein, as shown in Figure 1. The kit sensitivity is shown in Figure 2. This kit detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species. |
| 使用方法 | ELISA |
| 供应商 | CST |
| 背景 | Acetylation of the histone tail causes chromatin to adopt an "open" conformation, allowing increased accessibility of transcription factors to DNA. The identification of histone acetyltransferases (HATs) and their large multiprotein complexes has yielded important insights into how these enzymes regulate transcription (1,2). HAT complexes interact with sequence-specific activator proteins to target specific genes. In addition to histones, HATs can acetylate nonhistone proteins, suggesting multiple roles for these enzymes (3). In contrast, histone deacetylation promotes a "closed" chromatin conformation and typically leads to repression of gene activity (4). Mammalian histone deacetylases can be divided into three classes on the basis of their similarity to various yeast deacetylases (5). Class I proteins (HDACs 1, 2, 3, and 8) are related to the yeast Rpd3-like proteins, those in class II (HDACs 4, 5, 6, 7, 9, and 10) are related to yeast Hda1-like proteins, and class III proteins are related to the yeast protein Sir2. Inhibitors of HDAC activity are now being explored as potential therapeutic cancer agents (6,7). |
| 存放说明 | 4C |
| 参考文献 | Marmorstein, R. (2001) Cell Mol Life Sci 58, 693-703. Gregory, P.D. et al. (2001) Exp Cell Res 265, 195-202. Liu, Y. et al. (2000) Mol Cell Biol 20, 5540-53. Cress, W.D. and Seto, E. (2000) J Cell Physiol 184, 1-16. Gray, S.G. and Ekström, T.J. (2001) Exp Cell Res 262, 75-83. Thiagalingam, S. et al. (2003) Ann. N.Y. Acad. Sci. 983, 84-100. Vigushin, D.M. and Coombes, R.C. (2004) Curr Cancer Drug Targets 4, 205-18. |
Figure 1. Detection of HDAC4 protein in the various cells is examined by using the PathScan® Total HDAC4 Sandwich ELISA Kit. The absorbance readings at 450 nm are shown in the top figure, while the corresponding Western blot using | |
HDAC4 (D15C3) Rabbit mAb #7628 is shown in the bottom figure. |
