| 货号 | 73679C |
| 反应种属 | Human\Mouse |
| 目标/特异性 | PathScan® Total CD31 (PECAM-1) Sandwich ELISA Kit detects endogenous levels of CD31 (PECAM-1) protein in human cells, as shown in Figure 1. The kit sensitivity is shown in Figure 2. This kit detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species. |
| 使用方法 | ELISA |
| 供应商 | CST |
| 背景 | CD31 (Platelet Endothelial Cell Adhesion Molecule-1: PECAM-1), a member of the Ig superfamily of cell adhesion molecules, is expressed by circulating platelets, monocytes, neutrophils, some T cells, and endothelial cells and modulates cell adhesion, endothelial cell migration, and angiogenesis (1). CD31 is phosphorylated on Tyr686 at the cytoplasmic carboxy-terminal tail upon various stimuli (e.g. mechanical or oxidative stress), presumably by Src family members (2). The tyrosine phosphorylation mediates associations with a number of SH2 domain-containing binding partners such as PI3 kinase, SHIP, PLCγ, and SHP-2. Thus, CD31 serves as a scaffold for various signaling molecules (3). |
| 存放说明 | 4C |
| 参考文献 | Newman, P.J. (1997) J. Clin. Invest. 99, 3-8. Cao, M. Y. et al. (1998) J. Biol. Chem. 273, 15765-72. Ilan, N. and Madri, J.A. (2003) Curr. Opin. Cell Biol. 15, 515-24. |
Figure 1. Detection of CD31 (PECAM-1) protein in various cells is examined by using the PathScan® Total CD31 (PECAM-1) Sandwich ELISA Kit. The absorbance readings at 450 nm are shown in the top figure, while the corresponding | |
Western blot using CD31 (PECAM-1) (89C2) Mouse mAb #3528 is shown in the bottom figure. |
