| 货号 | 78222C |
| 反应种属 | Human |
| 目标/特异性 | PathScan® Phospho-SLP-76 (Ser376) Sandwich ELISA Kit detects endogenous levels of SLP-76 protein phoshorylated at Ser376 in human cells, as shown in Figure 1. The kit sensitivity is shown in Figure 2. This kit detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species. |
| 使用方法 | ELISA |
| 供应商 | CST |
| 背景 | SH2 domain-containing leukocyte protein of 76 kDa (SLP-76) is a hematopoietic adapter protein that is important in multiple biochemical signaling pathways and necessary for T cell development and activation (1). ZAP-70 phosphorylates SLP-76 and LAT as a result of TCR ligation. SLP-76 has amino-terminal tyrosine residues followed by a proline rich domain and a carboxy-terminal SH2 domain. Phosphorylation of Tyr113 and Tyr128 result in recruitment of the GEF Vav and the adapter protein Nck (2). TCR ligation also leads to phosphorylation of Tyr145, which mediates an association between SLP-76 and Itk, which is accomplished in part via the proline rich domain of SLP-76 and the SH3 domain of ITK (3). Furthermore, the proline rich domain of SLP-76 binds to the SH3 domains of Grb2-like adapter Gads (3,4). In resting cells, SLP-76 is predominantly in the cytosol. Upon TCR ligation, SLP-76 translocates to the plasma membrane and promotes the assembly of a multi-protein signaling complex that includes Vav, Nck, Itk and PLCγ1 (1). The expression of SLP-76 is tightly regulated; the protein is detected at very early stages of thymocyte development, increases as thymocyte maturation progresses, and is reduced as cells mature to CD4+ CD8+ double-positive thymocytes (5). |
| 存放说明 | 4C |
| 参考文献 | Clements, J.L. (2003) Immunol Rev 191, 211-9. Bubeck Wardenburg, J. et al. (1998) Immunity 9, 607-16. Bunnell, S.C. et al. (2000) J Biol Chem 275, 2219-30. Liu, S.K. et al. (1999) Curr Biol 9, 67-75. Clements, J.L. et al. (1998) J Immunol 161, 3880-9. |
Figure 2: The relationship between protein concentration of lysates from untreated and H2O2-treated Jurkat cells and the absorbance at 450 nm as detected by the PathScan® Phospho-SLP-76 Ser376 Sandwich ELISA Kit is shown. Unstarved Jurkat cells (0.5-1.0 x 106 cells/ml) were treated for 3 min with H2O2 (11 mM at 37°C), or left unteated, and then lysed with Cell Lysis Buffer (#9803). | |
Figure 1: Phospho-SLP-76 (Ser376) protein from untreated (-) and H2O2 treated (+) Jurkat cells is detected by PathScan® Phospho-SLP-76 (Ser376) Sandwich ELISA kit #78222. A lower signal of phospho-SLP-76 protein is seen in the untreated lysate. However, similar levels of total SLP-76 protein from both untreated and treated lysates are shown by Western analysis. The absorbance readings at 450 nm are shown in the top figure, while the corresponding western blots using SLP-76 Rabbit Antibody #4958 (left panel) or Phospho-SLP-76 (Ser376) (D9D6E) Rabbit mAb #14745 (right panel), are shown in the bottom figure. |
