| 货号 | 91959C |
| 反应种属 | Human\Rat\Moneky |
| 目标/特异性 | PathScan® Phospho-p90RSK (Thr359) Sandwich ELISA Kit detects endogenous levels of p90RSK protein phoshorylated at Thr359 in human cells, as shown in Figure 1. The kit sensitivity is shown in Figure 2. This kit detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species. |
| 使用方法 | ELISA |
| 供应商 | CST |
| 背景 | The 90 kDa ribosomal S6 kinases (RSK1-4) are a family of widely expressed Ser/Thr kinases characterized by two nonidentical, functional kinase domains (1) and a carboxy-terminal docking site for extracellular signal-regulated kinases (ERKs) (2). Several sites both within and outside of the RSK kinase domain, including Ser380, Thr359, Ser363, and Thr573, are important for kinase activation (3). RSK1-3 are activated via coordinated phosphorylation by MAPKs, autophosphorylation, and phosphoinositide-3-OH kinase (PI3K) in response to many growth factors, polypeptide hormones, and neurotransmitters (3). |
| 存放说明 | 4C |
| 参考文献 | Fisher, T.L. and Blenis, J. (1996) Mol Cell Biol 16, 1212-9. Smith, J.A. et al. (1999) J Biol Chem 274, 2893-8. Dalby, K.N. et al. (1998) J Biol Chem 273, 1496-505. |
Figure 1: Phospho-p90RSK (Thr359) protein from untreated (-) and TPA treated (+) HeLa cells is detected by PathScan® Phospho-p90RSK (Thr359) Sandwich ELISA kit. The absorbance readings at 450 nm are shown in the top figure, | |
while the corresponding Western blot using Phospho-p90RSK (Thr359) (D1E9) Rabbit mAb #8753 is shown in the bottom figure. |
