| 货号 | 99175C |
| 反应种属 | Mouse\Rat |
| 目标/特异性 | PathScan® Total Neurofilament-L Sandwich ELISA Kit detects endogenous levels of nerofilament-L protein in mouse or rat brains, as shown in Figure 1. The kit sensitivity is shown in Figure 2. This kit detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species. |
| 使用方法 | ELISA |
| 供应商 | CST |
| 背景 | The cytoskeleton consists of three types of cytosolic fibers: actin microfilaments, intermediate filaments, and microtubules. Neurofilaments are the major intermediate filaments found in neurons and consist of light (NFL), medium (NFM), and heavy (NFH) subunits (1). Similar in structure to other intermediate filament proteins, neurofilaments have a globular amino-terminal head, a central α-helical rod domain, and a carboxy-terminal tail. A heterotetrameric unit (NFL-NFM and NFL-NFH) forms a protofilament, with eight protofilaments comprising the typical 10 nm intermediate filament (2). While neurofilaments are critical for radial axon growth and determine axon caliber, microtubules are involved in axon elongation. PKA phosphorylates the head domain of NFL and NFM to inhibit neurofilament assembly (3,4). Research studies have shown neurofilament accumulations in many human neurological disorders including Parkinsons disease (in Lewy bodies along with α-synuclein), Alzheimers disease, Charcot-Marie-Tooth disease, and Amyotrophic Lateral Sclerosis (ALS) (1). |
| 存放说明 | 4C |
| 参考文献 | Al-Chalabi, A. and Miller, C.C. (2003) Bioessays 25, 346-55. Cohlberg, J.A. et al. (1995) J Biol Chem 270, 9334-9. Hisanaga, S. et al. (1994) Mol Biol Cell 5, 161-72. Sihag, R.K. et al. (1999) J Neurochem 72, 491-9. |
Figure 1. Detection of Neurofilament-L protein in mouse brain, HeLa cells, and rat brain is examined by using the PathScan® Total Neurofilament Sandwich ELISA Kit. The absorbance readings at 450 nm are shown in the top figure, while the corresponding western blot using Neurofilament-L (C28E10) Rabbit mAb #2837 is shown in the bottom figure. | |
Figure 2. The relationship between protein concentration of lysates from mouse brain and the absorbance at 450 nm as detected by the PathScan® Total Neurofilament-L Sandwich ELISA Kit is shown. Mouse brain was harvested and then lysed with the lysis buffer #9803. |
