| 货号 | 57046C |
| 反应种属 | Human |
| 目标/特异性 | PathScan® Phospho-YAP (Ser397) Sandwich ELISA Kit detects endogenous levels of YAP protein phoshorylated at Ser397 in human cells, as shown in Figure 1. The kit sensitivity is shown in Figure 2. This kit detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species. |
| 供应商 | CST |
| 背景 | YAP (Yes-associated protein, YAP65) was identified based on its ability to associate with the SH3 domain of Yes. It also binds to other SH3 domain-containing proteins such as Nck, Crk, Src, and Abl (1). In addition to the SH3 binding motif, YAP contains a PDZ interaction motif, a coiled-coil domain, and WW domains (2-4). While initial studies of YAP all pointed towards a role in anchoring and targeting to specific subcellular compartments, subsequent studies showed that YAP is a transcriptional co-activator by virtue of its WW domain interacting with the PY motif (PPxY) of the transcription factor PEBP2 and other transcription factors (5,6). In its capacity as a transcriptional co-activator, YAP is now widely recognized as a central mediator of the Hippo Pathway, which plays a fundamental and widely conserved role in regulating tissue growth and organ size. Phosphorylation at multiple sites (e.g., Ser109, Ser127) by LATS1/2 kinases promotes YAP translocation from the nucleus to the cytoplasm, where it is sequestered through association with 14-3-3 proteins in an Akt-dependent manner (7-9). These LATS-driven phosphorylation events serve to prime YAP for subsequent phosphorylation by CK1δ/ε in an adjacent phosphodegron, triggering proteosomal degradation of YAP (10). |
| 存放说明 | 4C |
| 参考文献 | 1 . Sudol, M. (1994) Oncogene 9, 2145-52. 2 . Mohler, P.J. et al. (1999) J Cell Biol 147, 879-90. 3 . Espanel, X. and Sudol, M. (2001) J Biol Chem 276, 14514-23. 4 . Sudol, M. et al. (1995) FEBS Lett 369, 67-71. 5 . Yagi, R. et al. (1999) EMBO J 18, 2551-62. 6 . Basu, S. et al. (2003) Mol Cell 11, 11-23. 7 . Zhao, B. et al. (2010) Genes Dev 24, 862-74. 8 . Zhao, B. et al. (2007) Genes Dev 21, 2747-61. 9 . Yu, F.X. et al. (2012) Cell 150, 780-91. 10 . Zhao, B. et al. (2010) Genes Dev 24, 72-85. |
