β-catenin (mouse) ELISA Kit
货号：
K3382-100 基本售价：
6189.5 元 规格：
100assays
产品信息
概述| 货号 | K3382-100 |
| 描述 | β-catenin (cadherin-associated protein beta, 88 kDa) is a dual function protein, regulating the coordination of cell–cell adhesion and gene transcription via the Wnt signaling pathway. Mutations and overexpression of β-catenin are associated with many cancers, including hepatocellular, colorectal, lung, breast, ovarian and endometrial cancers. BioVision’s mouse β-catenin ELISA Kit is based on the standard principle of a sandwich enzyme-linked immunosorbent assay. Mouse β-catenin antibody is coated on a 96-well plate. Standards and test samples are added to the wells and β-catenin present in a sample is bound by the immobilized antibody. An HRP-conjugate reagent is added subsequently. After washing away the unbound antibody/HRP conjugates, HRP substrate TMB is used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue product that changes into yellow after adding acidic stop solution. The density of yellow color is proportional to the mouse β-catenin captured onto the plate. This ELISA kit shows no species cross-reactivity. Detection Range: 3 – 180 ng/L. |
| 同种亚型 | Rabbit IgG |
| 来源宿主 | Rabbit |
性能| 供应商 | Biovision |
| 检测类型 | Absorbance (450 nm)• Species reactivity - mouse• Application- Quantitative detection of β-catenin, establishing normal range. |
| 样本类型以及上样量 | • Serum & plasma (EDTA/Citrate), urine and other biological fluids• Cell culture medium, tissue homogenates and cell lysates |
| 产品特色 | • Easy, convenient and time-saving method to assay for mouse β-catenin. • Detection Range: 3 – 180 ng/L. • This ELISA kit shows no species cross-reactivity. |
| 试剂盒组分 | • 96 wells coated with anti-mouse β-catenin antibody, 1 Microplate with 2 adhesive strips• Mouse β-catenin standard (270 ng/L)• HRP-conjugate reagent• Standard Diluent• Sample diluent• Chromogen Solution A• Chromogen Solution B• Stop Solution• Wash Solution (30x stock) |
| 运输条件 | Blue Ice |
| 存放说明 | +4℃ |
