β-catenin (human) ELISA Kit
货号：
K3381-100 基本售价：
6189.5 元 规格：
100assays
产品信息
概述| 货号 | K3381-100 |
| 描述 | β-catenin (cadherin-associated protein beta, 88 kDa) is a dual function protein, regulating the coordination of cell–cell adhesion and gene transcription via the Wnt signaling pathway. Mutations and overexpression of β-catenin are associated with many cancers, including hepatocellular, colorectal, lung, breast, ovarian and endometrial cancers. BioVision’s human β-catenin ELISA Kit is based on the standard principle of a sandwich enzyme-linked immunosorbent assay. Human β-catenin antibody is coated on a 96-well plate. Standards and test samples are added to the wells and β-catenin present in a sample is bound by the immobilized antibody. An HRP-conjugate reagent is added subsequently. After washing away the unbound antibody/HRP conjugates, HRP substrate TMB is used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue product that changes into yellow after adding acidic stop solution. The density of yellow color is proportional to the human β-catenin captured onto the plate. This ELISA kit shows no species cross-reactivity. Detection Range: 0.156 – 10 ng/ml. |
| 同种亚型 | Rabbit IgG |
| 来源宿主 | Rabbit |
性能| 供应商 | Biovision |
| 检测类型 | Absorbance (450 nm)• Species reactivity - human• Application- Quantitative detection of β-catenin, establishing normal range. |
| 样本类型以及上样量 | • Serum and other biological fluids• Cell culture medium, tissue homogenates and cell lysates |
| 产品特色 | • Easy, convenient and time-saving method to assay for human β-catenin. • Detection Range: 0.156 – 10 ng/ml. • This ELISA kit shows no species cross-reactivity. |
| 试剂盒组分 | • 96 wells coated with anti-human β-catenin antibody, 1 Microplate • Human β-catenin standard (18 ng/ml)• HRP-conjugate reagent• Standard Diluent• Sample diluent• Chromogen Solution A• Chromogen Solution B• Stop Solution• Wash Solution (30x stock)• Sealed Bag• Plate cover |
| 运输条件 | Blue Ice |
| 存放说明 | +4℃ |
