Apolipoprotein E (human) ELISA Kit
货号：
K4696-100 基本售价：
5826.5 元 规格：
100assays
产品信息
概述| 货号 | K4696-100 |
| 描述 | BioVision’s Human Apolipoprotein E (ApoE) Enzyme-Linked Immunosorbent Assay (ELISA) Kit is an in vitro assay for the quantitative measurement of human ApoE. ApoE transports lipoproteins, fat-soluble vitamins, and cholesterol via the lymph system to the blood. It is synthesized mainly in liver, while it has also been identified in the brain, kidneys, and spleen. In the nervous system, ApoE is synthesized in non-neuronal cell types, most notably astroglia and microglia, while neurons preferentially express its receptors. Defects in ApoE lead to familial dysbetalipoproteinemia (increased plasma cholesterol and triglycerides). More recently, ApoE has been implicated in several biological processes not directly related to lipoprotein transport, including Alzheimers disease, immunoregulation, and cognition. The assay employs an antibody specific for human ApoE coated on a 96-well plate. Standard and samples are pipetted into wells and ApoE present in the sample is bound to the wells by immobilized antibody. Wells are washed and human ApoE specific detection antibody is added. After washing away unbound detection antibody, HRP-conjugate is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells, and color develops in proportion to the amount of bound ApoE. Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. This ELISA kit recognizes ApoE-2, ApoE-3, and ApoE-4 isoforms. Sensitivity of the kit is 25 ng/ml and detection range is from 25 ng to 1.6 µg/ml. Recovery inside this range is between 86 and 111% (average recovery is 102%). The intra-assay reproducibility as measured by the coefficient of variation (CV) is < 8 % & inter-assay has CV < 12 %. |
| 同种亚型 | Rabbit IgG |
| 来源宿主 | Rabbit |
性能| 供应商 | Biovision |
| 检测类型 | Absorbance (450 nm)• Species reactivity - human• Applications - Quantitative measurement of human Apolipoprotein E (ApoE) |
| 样本类型以及上样量 | • Serum and plasma• Cell lysate and cell culture supernatant• Cerebrospinal Fluid (CSF) |
| 产品特色 | • Easy, convenient and time-saving method to assay for human ApoE. • Sensitivity: 25 ng/ml. Detection range: 25 ng to 1.6 µg/ml. |
| 试剂盒组分 | • Plate Coated with ApoE Ab• Assay Diluent• Wash Buffer A (10x)• Wash Buffer B (10x)• rh ApoE Standard• Detection Antibody (100x)• HRP Conjugate (100x)• TMB Substrate • Stop Solution • Plate Sealer |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |
