| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. HIV-1 reduces Abeta-degrading enzymatic activities in primary human mononuclear phagocytes.
Authors: Lan X, Xu J, Kiyota T, Peng H, Zheng JC, Ikezu T
J. Immunol., 2011;186(12):6925-32.
Species: Human
Sample Type: Cell Lysates
Expression and functional profiling of neprilysin, insulin-degrading enzyme, and endothelin-converting enzyme in prospectively studied elderly and Alzheimer's brain.
Authors: Wang S, Wang R, Chen L, Bennett DA, Dickson DW, Wang DS
J. Neurochem., 2010;115(1):47-57.
Species: Human
Sample Type: Tissue Homogenates
Intra- versus extracellular effects of microglia-derived cysteine proteases in a conditioned medium transfer model.
Authors: Wendt W, Schulten R, Stichel CC, Lubbert H
J. Neurochem., 2009;110(6):1931-41.
Species: Mouse
Sample Type: Cell Lysates
Immunocapture-based fluorometric assay for the measurement of neprilysin-specific enzyme activity in brain tissue homogenates and cerebrospinal fluid.
Authors: Miners JS, Verbeek MM, Rikkert MO, Kehoe PG, Love S
J. Neurosci. Methods, 2008;167(2):229-36.
Species: Human
Sample Type: Tissue Homogenates
ACE mediates ventilator-induced lung injury in rats via angiotensin II but not bradykinin.
Authors: Wosten-van Asperen RM, Lutter R, Haitsma JJ, Merkus MP, van Woensel JB, van der Loos CM, Florquin S, Lachmann B, Bos AP
Eur. Respir. J., 2007;31(2):363-71.
Species: Rat
Sample Type: BALF
Human Opiorphin, a natural antinociceptive modulator of opioid-dependent pathways.
Authors: Wisner A, Dufour E, Messaoudi M, Nejdi A, Marcel A, Ungeheuer MN, Rougeot C
Proc. Natl. Acad. Sci. U.S.A., 2006;103(47):17979-84.
Characterization of insulin degrading enzyme and other amyloid-beta degrading proteases in human serum: a role in Alzheimer's disease?
Authors: Liu Z, Zhu H, Fang G, Walsh K, Mwamburi M, Wolozin B, Abdul-Hay S, Ikezu T, Leissring M, Qiu W
Oncogene, 0;29(2):329-40.
Species: Human
Sample Type: Serum
Application: Substrate for Enzyme Digestion |
| 生物活性 | Thepeptide substrate contains a highly fluorescent 7-methoxycoumarin group that isefficiently quenched by resonance energy transfer to the 2,4-dinitrophenylgroup. It can be used to measure the activities of peptidases that are capableof cleaving an amide bond between the fluorescent group and the quencher group,causing an increase in fluorescence. Itis an excellent substrate for endothelin-converting enzyme-1 (ECE-1) andneprilysin. The cleavage site by ECE-1 is the peptide bond between Ala and Phe(Johnson, G.D. and Ahn, K., 2000, Anal. Biochem. 286:112). The substrate isderived from bradykinin. It is also an excellent substrate for ACE and activeCathepsin A and X/Z/P. |
