产品中心
当前位置：首页>产品中心Anti-Gamma-Adaptin
货号： bs-1600R 基本售价： 1380.0 元 规格： 100ul
- 规格：100ul
- 价格：1380.00元
- 规格：200ul
- 价格：2200.00元
产品信息
- 产品编号
- bs-1600R
- 英文名称
- Gamma-Adaptin
- 中文名称
- 衔接蛋白γ/γ-Adaptin抗体
- 别 名
- Adapter-related protein complex 1 subunit gamma-1; Adaptor protein complex AP 1 gamma 1 subunit; Adaptor protein complex AP 1 subunit gamma 1; Adaptor protein complex AP-1 subunit gamma-1; Adaptor related protein complex 1 gamma 1 subunit; adaptor-related protein complex 1 gamma 1 subunit; ADTG; ADTG; AP 1 complex subunit gamma 1; AP-1 complex subunit gamma-1; AP1G1; AP1G1_HUMAN; CLAPG1; CLAPG1; Clathrin assembly protein complex 1 gamma 1 large chain; Clathrin assembly protein complex 1 gamma large chain; Clathrin assembly protein complex 1 gamma-1 large chain; Clathrin associated/assembly/adaptor protein large gamma 1; clathrin-associated/assembly/adaptor protein large gamma 1; Gamma1 adaptin; Gamma1-adaptin; Golgi adaptor HA1/AP1 adaptin gamma subunit; Golgi adaptor HA1/AP1 adaptin subunit gamma 1; Golgi adaptor HA1/AP1 adaptin subunit gamma-1; MGC18255.
- 规格价格
- 100ul/1380元购买 200ul/2200元购买 大包装/询价
- 说 明 书
- 100ul 200ul
- 研究领域
- 肿瘤 免疫学 信号转导
- 抗体来源
- Rabbit
- 克隆类型
- Polyclonal
- 交叉反应
- Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Rabbit,
- 产品应用
- WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
- 分 子 量
- 91kDa
- 细胞定位
- 细胞浆 细胞膜
- 性 状
- Lyophilized or Liquid
- 浓 度
- 1mg/ml
- 免 疫 原
- KLH conjugated synthetic peptide derived from human Gamma-Adaptin:721-822/822
- 亚 型
- IgG
- 纯化方法
- affinity purified by Protein A
- 储 存 液
- 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
- 保存条件
- Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
- PubMed
- PubMed
- 产品介绍
- background:
Adaptins are important components of clathrin-coated vesicles transporting ligand-receptor complexes from the plasma membrane or from the trans-Golgi network to lysosomes. The adaptin family of proteins is composed of four classes of molecules named alpha, beta, beta prime and gamma adaptins. Adaptins, together with medium and small subunits, form a heterotetrameric complex called an adaptor, whose role is to promote the formation of clathrin-coated pits and vesicles. The protein encoded by this gene is a gamma-adaptin protein and it belongs to the adaptor complexes large subunits family.
Function:
Subunit of clathrin-associated adaptor protein complex 1 that plays a role in protein sorting in the late-Golgi/trans-Golgi network (TGN) and/or endosomes. The AP complexes mediate both the recruitment of clathrin to membranes and the recognition of sorting signals within the cytosolic tails of transmembrane cargo molecules.
Subunit:
Adaptor protein complex 1 (AP-1) is an heterotetramer composed of two large adaptins (gamma-type subunit AP1G1 and beta-type subunit AP1B1), a medium adaptin (mu-type subunit AP1M1 or AP1M2) and a small adaptin (sigma-type subunit AP1S1 or AP1S2 or AP1S3). Binds RABEP1 and SYNRG. Interacts (via GAE domain) with AP1AR (via coiled-coil domain).
Subcellular Location:
Golgi apparatus. Cytoplasmic vesicle, clathrin-coated vesicle membrane; Peripheral membrane protein; Cytoplasmic side.
Tissue Specificity:
Widely expressed.
Similarity:
Belongs to the adaptor complexes large subunit family.
Contains 1 GAE domain.
SWISS:
P22892
Gene ID:
164
Database links:Entrez Gene: 164 Human
Entrez Gene: 11765 Mouse
Entrez Gene: 171494 Rat
Omim: 603533 Human
SwissProt: O43747 Human
SwissProt: P22892 Mouse
Unigene: 461253 Human
Unigene: 37210 Mouse
Unigene: 430782 Mouse
Unigene: 471663 Mouse
Unigene: 9446 Rat
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
γ-Adaptin参与披网格蛋白小泡组装的一种蛋白质, 分子量为100kDa, 在披网格蛋白小泡组装中与受体的细胞质结构域相互作用, 起衔接作用。有两种类型衔接蛋白, AP1参与反面高尔基体的披网格蛋白小泡的组装, AP2则参与从细胞质膜形成的披网格蛋白的组装。
- 产品图片
Sample:
293T(Human) Cell Lysate at 30 ug
Jurkat(Human) Cell Lysate at 30 ug
Primary: Anti-Gamma-Adaptin (bs-1600R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 91 kD
Observed band size: 91 kD
Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Gamma-Adaptin) Polyclonal Antibody, Unconjugated (bs-1600R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Gamma-Adaptin Polyclonal Antibody, Unconjugated(bs-1600R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Tissue/cell: rat lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Gamma-Adaptin Polyclonal Antibody, Unconjugated(bs-1600R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Tissue/cell: Rat brian; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti- Gamma-Adaptin, Unconjugated(bs-1600R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Cell culture(MCF7); Paraformaldehyde-fixed; Incubated by 0.3% Triton-100 for 20 min; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Gamma-Adaptin) Polyclonal Antibody, Unconjugated (bs-1600R) at 1:200 overnight at 4°C, followed by a conjugated secondary antibody (bs-0295G-FITC) for 90 minutes and DAPI staining.
Cell culture(SW480); Paraformaldehyde-fixed; Incubated by 0.3% Triton-100 for 20 min; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Gamma-Adaptin) Polyclonal Antibody, Unconjugated (bs-1600R) at 1:200 overnight at 4°C, followed by a conjugated secondary antibody (bs-0295G-FITC) for 90 minutes and DAPI staining.