性能| 供应商 | R&D Systems |
| 内毒素水平 | <1.0 EU per 1 μg of the protein by the LAL method. |
| 生物活性 | Measured by its ability to induce IL-6 secretion by NIH‑3T3 mouse embryonic fibroblast cells. The ED50 for this effect is typically 0.4‑2 ng/mL. |
| 溶解方法 | Reconstitute at 100 μg/mL in 4 mM HCl containing at least 0.1% human or bovine serum albumin |
| 背景 | Human IL-17A/F is an approximately 40 kDa, secreted, disulfide-linked heterodimeric glycoprotein comprised of two members of the IL-17 family of cytokines, IL-17A and IL-17F (1, 2). Members of this family demonstrate a structural motif termed a cysteine knot that also characterizes a large superfamily of growth factors. Although most cysteine knot superfamily members use three intrachain disulfide bonds to create a knot, IL-17 family molecules generate the same structural form with only two disulfide links (3-5). Mature human IL-17A and IL-17F share 61% and 56% amino acid sequence identity with mouse IL-17A and IL-17F, respectively. They share 50% aa sequence identity with each other. IL-17A/F and the IL-17A and IL-17F homodimers are produced by IL-23 activated Th17 cells (1, 6-10). The widely expressed receptors IL-17 RA and IL-17 RC form a heterodimer for the binding of IL-17A and IL-17F, as well as the heterodimeric IL-17A/F (6, 11, 12). IL-17A/F is a biologically active protein that induces chemokine production and airway neutrophilia with intermediate potency between IL-17A (most potent) and IL-17F (least potent) (7, 12). It is up-regulated in immune cells during inflammatory arthritis and contributes to disease severity (13). |
| 运输条件 | Blue Ice |
N-terminal
Sequence Analysis | Gly24(IL-17A) & Arg21(IL-17F) |
| 预期分子量 | 15 (unlabeled IL-17A), 15 (unlabeled IL-17F) kDa |
| 存放说明 | -20℃ |
| 纯度 | >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining. |
| 不含载体 | What does CF mean?
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA. What formulation is right for me?
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere. |
| 参考文献 | - Wright, J.F. et al. (2007) J. Biol. Chem. 282:13447.
- Chang, S.H. and C. Dong (2007) Cell Res. 17:435.
- Kawaguchi, M. et al. (2004) J. Allergy Clin. Immunol. 114:1265.
- Kolls, J.K. and A. Linden (2004) Immunity 21:467.
- Gu, C. et al. (2013) Cytokine 64:477.
- Cheung, P.F.Y. et al. (2008) J. Immunol. 180:5625.
- Liang, S.C. et al. (2007) J. Immunol. 179:7791.
- Ouyang, W. et al. (2008) Immunity 28:454.
- Steinman, L. (2007) Nat. Med. 13:139.
- Hunter, C.A. (2005) Nat. Rev. Immunol.5:521.
- Kuestner, R.E. et al. (2007) J. Immunol. 179:5462.
- Wright, J.F. et al. (2008) J. Immunol. 181:2799.
- Sarkar, S. et al. (2014) Clin. Exp. Immunol. 177:652.
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