Recombinant Human R-Spondin 1 Biotinylated Protein, CF (25 UG)【科瑞奥博】

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Recombinant Human R-Spondin 1 Biotinylated Protein, CF (25 UG)

货号： BT4645-025/CF 基本售价： 5809.9 元规格： 25 ug

产品信息

概述
货号	BT4645-025/CF
别名	Cristin 3; CRISTIN3; FLJ40906Roof plate-specific spondin-1; hRspo1; roof plate-specific spondin; RSPO; RSPO1; R-spondin homolog (Xenopus laevis); RSPONDIN; R-spondin1; R-spondin-1
全称	Roof Plate-specific Spondin 1
反应种属	Human
来源	Chinese Hamster Ovary cell line, CHO-derived Arg31-Ala263
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	284654 (Human); 192199 (Mouse)
内毒素水平	<0.10 EU per 1 μg of the protein by the LAL method.
生物活性	Measured by its ability to induce Topflash reporter activity in HEK293T human embryonic kidney cells. The ED₅₀ for this effect is typically 5-30 ng/mL in the presence of 5 ng/mL recombinant mouse Wnt-3a.
溶解方法	Reconstitute at 100 μg/mL in PBS.
背景	R-Spondin 1 (RSPO1), also known as Cristin 3, is a 27 kDa secreted protein in the R-Spondin family of Wnt/ beta -catenin signaling regulators (1). These proteins contain two adjacent cysteine-rich furin-like domains followed by a thrombospondin (TSP-1) motif and a region rich in basic residues. Mature human R-Spondin 1 shares 87% amino acid sequence identity with mouse and rat R-Spondin 1 (2). Alternative splicing generates additional isoforms that have a substituted N-terminus or lack the TSP-1 domain. R-Spondin 1 enhances canonical Wnt/ beta -catenin signaling by competing with the Wnt antagonist Dkk-1, binding to Frizzled-8, Kremen, LRP-6, Lgr4, Lgr5, and Lgr6, and enhancing cell surface availability of Wnt receptors (3-11). R-Spondin 1 functions in dorsal neural tube development (12) as well as male and female germ cell development (7, 10, 13). It also induces bone formation (6), intestinal crypt cell proliferation (14), angiogenesis (7, 15), and insulin secretion from pancreatic beta cells (11).
Accession #	Q2MKA7
运输条件	Blue Ice
N-terminal Sequence Analysis	Arg31
预期分子量	26 (unlabeled) kDa
存放说明	-20℃
纯度	>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
不含载体	What does CF mean? CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA. What formulation is right for me? In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
参考文献	Jin, Y.R. and J.K. Yoon (2012) Int. J. Biochem. Cell Biol. 44:2278. Chen, J-Z. et al. (2002) Mol. Biol. Rep. 29:287. Binnerts, M.E. et al. (2007) Proc. Natl. Acad. Sci. USA 104:14700. Nam, J.-S. et al. (2006) J. Biol. Chem. 281:13247. Wei, Q. et al. (2007) J. Biol. Chem. 282:15903. Kronke, G. et al. (2010) Arthritis Rheum. 62:2303. Caruso, M. et al. (2015) PLoS One 10:e0124213. Hao, H.-X. et al. (2012) Nature 485:195. de Lau, W. et al. (2011) Nature 476:293. Chassot, A.-A. et al. (2011) PLoS One 6:e25641. Wong, V.S.C et al. (2010) J. Biol. Chem. 285:21292. Kamata, T. et al. (2004) Biochim. Biophys. Acta 1676:51. Chassost, A.-A. et al. (2012) Development 139:4461. Kim, K.-A. et al. (2005) Science 309:1256. Gore, A.V. et al. (2011) Development 138:4875.

参考图片

Both Biotinylated Recombinant Human R-Spondin 1 (Catalog # BT4645/CF) and unlabeled Recombinant Human R-Spondin 1 (Catalog #
4645-RS) induces activation of beta -catenin response in Topflash Luciferase assay using HEK293T human embryonic kidney cells. The ED₅₀ for this effect is typically 5-30 ng/mL in the presence of 5 ng/mL Recombinant Mouse Wnt-3a (Catalog # 1324-WN). The similarity in activity highlights that the biotinylated protein is fully functional.