Recombinant Viral B8R Biotinylated Protein, CF (25 UG)【科瑞奥博】

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Recombinant Viral B8R Biotinylated Protein, CF (25 UG)

货号： BT8225-025 基本售价： 5809.9 元规格： 25 ug

产品信息

概述
货号	BT8225-025
别名	B8R
全称	Soluble Interferon alpha/beta Receptor B8
来源	Chinese Hamster Ovary cell line, CHO-derived Lys18-Ser272, with a C-terminal 6-His tag
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	3707661 (Viral)
内毒素水平	<0.10 EU per 1 μg of the protein by the LAL method.
生物活性	Measured by its ability to inhibit rhIFN-gamma mediated protection of HeLa human cervical epithelial carcinoma cells to viral lysis. Meager, A. (1987) in Lymphokines and Interferons, a Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 129. Th ED₅₀ for this effect is typically 0.5-3 ng/mL.
溶解方法	Reconstitute at 250 μg/mL in PBS.
背景	B8R is a secreted Interferon gamma (IFN-gamma ) binding protein encoded by the open reading frame of the Vaccina virus (smallpox) (1, 2). B8R is a 43 kDa protein and shares only 19% amino acid sequence identity with the extracellular region of the human IFN-gamma Receptor 1 (IFN-gamma R1). However, B8R binds IFN-gamma orthologs from multiple species including human, rat, rabbit, bovine, and chicken with high affinity, but it binds mouse IFN-gamma with low affinity (1, 3, 4). During infection, virally-induced B8R binds and sequesters endogenous IFN-gamma, thereby suppressing the host immune response and promoting viral immune evasion (5). While B8R is not required for viral replication, it contributes significantly to Vacciniavirus virulence and is frequently inactivated prior to Vaccinia use in vaccines (5-9). IFN-gamma peptide mimetics that activate IFN-gamma R1 but are not bound and sequestered by B8R have been developed for research and therapeutic use (10, 11).
Accession #	P24770
运输条件	Blue Ice
N-terminal Sequence Analysis	Lys18
预期分子量	30 (unlabeled) kDa
存放说明	-20℃
纯度	>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
参考文献	Alcami, A. and G.L. Smith (1995) J. Virol. 69:4633. Goebel, S.J. et al. (1990) Virology 179:247. Puehler, F. et al. (1998) Virology 248:231. Mossman, K. et al. (1995) Virology 208:762. Smith, G.L. et al. (2013) J. Gen. Virol. 94:2367. Symons, J.A. et al. (2002) J. Gen. Virol. 83:1953. Verardi, P.H. et al. (2001) J. Virol. 75:11. Denes, B. et al. (2006) J. Gene Med. 8:814. Dimier, J. et al. (2011) J. Virol. 85:5016. Ahmed, C.M. et al. (2007) J. Immunol. 178:4576. Ahmed, C.M. et al. (2005) J. Virol. 79:5632.

参考图片

Both Biotinylated Recombinant Viral B8R (Catalog # BT8225) and unlabeled Recombinant Viral B8R (Catalog # 8225-BR) inhibits IFN-gamma activity. The viral receptor B8R inhibits IFN-gamma-mediated protection of HeLa human cervical epithelial carcinoma cells to viral lysis. The ED₅₀ for this effect is typically 0.5-3 ng/mL. The similarity in activity highlights that the biotinylated protein is fully functional.