Recombinant Human GAPDH Protein
货号:
NBP2-52615 基本售价:
5641.3 元 规格:
100 ug
产品信息
概述货号 | NBP2-52615 |
反应种属 | HuSpecies Glossary |
应用 | PAGE |
性能供应商 | Novus |
背景 | Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is a catalytic enzyme commonly known to be involved in glycolysis. The enzyme exists as a tetramer composed of 36-kDa subunits and has various intracellular functions. GAPDH catalyzes the reversible reduction of 1,3-bisphosphoglycerate to glyceraldehyde 3-phosphophate in the presence of NADPH. Besides functioning as a glycolytic enzyme in cytoplasm, evidence suggests that mammalian GAPDH is also involved in a great number of intracellular processes such as membrane fusion, microtubule bundling, phosphotransferase activity, nuclear RNA export, DNA replication and DNA repair. Recombinant GAPDH protein was expressed in E.coli and purified by using conventional chromatography techniques. |
存放说明 | Store at 4C short term. Aliquot and store at -70C long term. Avoid freeze-thaw cycles. |
存储溶液 | Liquid. In 20 mM Tris-HCl buffer (pH 8.0) containing 1 mM DTT, 1 mM EDTA, 20% glycerol |
浓度 | 1 mg/ml |
纯度 | >95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain |