| 货号 | BT5439-010/CF |
| 别名 | dickkopf (Xenopus laevis) homolog 1; dickkopf homolog 1 (Xenopus laevis); dickkopf related protein-1; Dickkopf-1; dickkopf-related protein 1; DKK-1; hDkk-1; SKdickkopf-1 like | 全称 | Dickkopf-1 |
| 反应种属 | Human |
| 来源 | Spodoptera frugiperda,Sf 21 (baculovirus)-derived Thr32-His266 Accession # O94907 |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 22943 (Human); 13380 (Mouse) |
| 内毒素水平 | <1.0 EU per 1 μg of the protein by the LAL method. |
| 生物活性 | Measured by its ability to inhibit Wnt-3a-induced alkaline phosphatase production by MC3T3‑E1 mouse preosteoblast cells. The ED50 for this effect is typically 0.2-1 μg/ml in the presence of 10 ng/ml of Recombinant Mouse Wnt-3a (Catalog # 1324-WN). |
| 溶解方法 | Reconstitute at 100 μg/mL in PBS. |
| 背景 | Dickkopf related protein 1 (Dkk-1) is the founding member of the Dickkopf family of proteins that includes Dkk-1, -2, -3, -4, and a related protein, Soggy (1, 2). Dkk proteins are secreted proteins that contain two conserved cysteine-rich domains (CRDs) separated by a linker region. Each domain contains ten cysteine residues (1-3). Mature human Dkk-1 is a 40 kDa glycosylated protein that shares 83% amino acid sequence identity with mouse and rat Dkk-1. Dkk-1 is a well-characterized antagonist of Wnt/beta-Catenin signaling (1, 2, 4, 5). This pathway is activated by Wnt engagement of a receptor complex composed of the Frizzled proteins and one of two low-density lipoprotein receptor-related proteins, LRP-5 or LRP-6 (4). Crystallographic studies have shown that Dkk-1 interacts with LRP-6 as a bipartite inhibitor, with both CRDs binding the extracellular domain of LRP-6 simultaneously (4, 6-8). Dkk-1/LRP-6/Krm2 complex internalization has been shown to down-regulate Wnt signaling (4, 9). Dkk-1 is expressed throughout development and antagonizes Wnt-7a during limb development (10, 11). Other sites of expression include developing neurons, hair follicles, and the retina (12, 13). The balance between Wnt signaling and Dkk-1 inhibition is critical for bone formation and homeostasis (14). Insufficient or excess Dkk-1 activity in bone results in increased or decreased bone density, respectively (12, 15). In adults, Dkk-1 is expressed in osteoblasts and osteocytes, and neurons. Cerebral ischemia induces Dkk-1 expression, which contributes to neuronal cell death (16). Dkk-1 also likely has a complex role in cancer, as it has been shown to act as a tumor suppressor and also to promote metastasis (17-19). |
| Accession # | O94907 |
| 运输条件 | Blue Ice |
| N-terminal Sequence Analysis | Thr32 |
| 预期分子量 | 26 (unlabeled) kDa |
| SDS-PAGE | 33-38 kDa, reducing conditions |
| 存放说明 | -20℃ |
| 纯度 | >95%, by SDS-PAGE with silver staining |
| 不含载体 | What does CF mean? CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA. What formulation is right for me? |
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