| 货号 | 3575-KS-010 |
| 别名 | Apoptosis signal-regulating kinase 1; ASK-1; ASK1MEKK 5; EC 2.7.11; MAP/ERK kinase kinase 5; MAP3K5; MAPK/ERK kinase kinase 5; MAPKKK5EC 2.7.11.25; MEK kinase 5; MEKK5; MEKK5apoptosis signal regulating kinase 1; mitogen-activated protein kinase kinase kinase 5 | 全称 | Apoptosis Signal-regulated Kinase 1 |
| 反应种属 | Human |
| 来源 | Spodoptera frugiperda,Sf 9 (baculovirus)-derived aa 649-946 Accession # NM_005923 |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 4217 (Human); 26408 (Mouse) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. TGF-beta-activated kinase 1 (TAK1) and apoptosis signal-regulating kinase 1 (ASK1) interact with the promyogenic receptor Cdo to promote myogenic differentiation via activation of p38MAPK pathway. | |
| 内毒素水平 | <1.0 EU per 1 μg of the protein by the LAL method. |
| 生物活性 | The activity of ASK1 is typically 82-112 nmol/min/mg using a myelin basic protein (MBP) substrate (see Activity Assay Protocol). |
| 背景 | ASK1, also known as MEKK5 or MAPKKK5, activates MKK3, MKK4 (SEK1), and MKK6. Over-expression of ASK1 induces apoptotic cell death and is activated in cells treated with tumor necrosis factor alpha (TNF ) (1). ASK1 interacts with members of the TRAF family and is activated by TRAF2 in the TNF-signaling pathway. After activation by TRAF2, ASK1 activates MKK4, which in turn activates JNK. Thus, ASK1 is a mediator of TRAF2-induced JNK activation (2). |
| Accession # | NM_005923 |
| 运输条件 | Dry Ice |
| N-terminal Sequence Analysis | Using an N-terminal GST tag |
| 预期分子量 | 60 kDa |
| 存放说明 | -80℃ |
| 纯度 | >70%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 μg per lane |
| 参考文献 |
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The approximate molecular weight is 60 kDa and the average purity is 90%. |
