| 货号 | 4587-KS-010 |
| 别名 | EC 2.7.10; EC 2.7.10.2; EMT; EMTMGC126257; homolog of mouse T-cell itk/tsk; IL2-inducible T-cell kinase; Kinase EMT; LYK; LYKMGC126258; PSCTK2; T-cell-specific kinase; Tcsk; tyrosine-protein kinase ITK/TSK; Tyrosine-protein kinase Lyk | 全称 | IL-2-inducible T-cell Kinase |
| 反应种属 | Human |
| 来源 | Spodoptera frugiperda,Sf 9 (baculovirus)-derived aa 352-620 Accession # NM_005546 |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 3702 (Human); 16428 (Mouse); 363577 (Rat) |
| 生物活性 | The activity of ITK is typically 49-67 nmol/min/mg using a myelin basic protein (MBP) substrate (see Activity Assay Protocol). |
| 背景 | ITK is a member of the TEC family of non-receptor tyrosine kinases. ITK is expressed in T cells and is important for T cell development and activation through the antigen receptor. ITK requires prior activation of Lck, ZAP70, and PI3-kinase for efficient activation and shares major substrates with both Lck and ZAP70 (1). ITK knockout mice show multiple effects on T cell development, cytokine production, and T-helper cell differentiation. T cells that lack or express mutant versions of ITK show impaired TCR-induced actin polymerization, cell polarization, and regulation of the signaling events involved in cytoskeletal reorganization (2). |
| Accession # | NM_005546 |
| 运输条件 | Dry Ice |
| N-terminal Sequence Analysis | Using an N-terminal GST tag |
| SDS-PAGE | 53 kDa |
| 存放说明 | -80℃ |
| 纯度 | >70%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 μg per lane |
| 参考文献 |
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The approximate molecular weight is 53 kDa and the average purity is 90%. |
