概述| 货号 | 3200-KS-010 |
| 别名 | EC 2.7.11; EC 2.7.11.1; serine/threonine protein kinase SGK; serine/threonine-protein kinase Sgk1; serum/glucocorticoid regulated kinase 1; serum/glucocorticoid regulated kinase; Serum/glucocorticoid-regulated kinase 1; SGK |
全称 | Serum/Glucocorticoid Regulated Kinase 1 | | 反应种属 | Human |
| 来源 | Spodoptera frugiperda,Sf 9 (baculovirus)-derived aa 60-431 Accession # NM_005627 With an N-terminal GST tag |
| 产品组分 | |
性能| 供应商 | R&D Systems |
| Entrez Gene IDs | 6446 (Human); 20393 (Mouse); 29517 (Rat) |
| 生物活性 | The activity of SGK1 is typically 99-135 nmol/min/mg using a synthetic peptide substrate (RPRAATF) (see Activity Assay Protocol). |
| 背景 | SGK1 is a member of the serum- and glucocorticoid-induced protein kinase that is activated in vitro by 3-phosphoinositide-dependent protein kinase-1 (PDK 1) and in vivo in response to signals that activate phosphatidylinositol (PI) 3-kinase (1). SGK1 mRNA is expressed in all tissues and the level of SGK1 mRNA is increased by stimulation with serum or dexamethasone. SGK1 promotes cell survival by phosphorylating and inactivating FKHRL1 (2). SGK and Akt display differences with respect to the efficacy with which they phosphorylate the three regulatory sites on FKHRL1. |
| Accession # | NM_005627 |
| 运输条件 | Dry Ice |
| SDS-PAGE | 73 kDa |
| 存放说明 | -80℃ |
| 纯度 | >70%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 μg per lane |
| 参考文献 | - Kobayashii, T. et al. (1999) Biochem. J. 1:189.
- Brunet, A. et al. (2001) Mol. Cell. Biol. 21:952.
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参考图片The approximate molecular weight is 73 kDa and the average purity is 95%. |
