| 货号 | 864-KS-010 |
| 别名 | [Pyruvate dehydrogenase [lipoamide]] kinase isozyme 1, mitochondrial; EC 2.7.11; EC 2.7.11.2; mitochondrial pyruvate dehydrogenase, lipoamide, kinase isoenzyme 1; PDPK1; PKB kinase; Pyruvate dehydrogenase kinase isoform 1; pyruvate dehydrogenase kinase, isoenzyme 1; pyruvate dehydrogenase kinase, isozyme 1 | 全称 | Phosphoinositide Dependent Kinase-1 |
| 反应种属 | Human |
| 来源 | Spodoptera frugiperda,Sf 9 (baculovirus)-derived |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 5170 (Human); 18607 (Mouse); 81745 (Rat) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. The Akt activation inhibitor TCN-P inhibits Akt phosphorylation by binding to the PH domain of Akt and blocking its recruitment to the plasma membrane. | |
| 生物活性 | The activity of PDK-1 is typically 102-138 nmol/min/mg using a synthetic peptide substrate (see Activity Assay Protocol). |
| 背景 | PDK-1 (3-phosphoinositide-dependent protein kinase) is activated by the presence of PtdIns(3,4,5)P3 or PtdIns(3,4)P2 (1). PDK‑1 then activates protein kinase B (PKB) that, in turn, inactivates glycogen synthase kinase-3 (GSK-3). The phosphorylation of other proteins by PKB and GSK-3 is likely to mediate many of the intracellular actions of insulin. Thus, PDK‑1 plays a key role in mediating many of the actions of the second messenger(s) PtdIns(3,4,5)P3 and/or PtdIns(3,4)P2. Human PDK‑1 is a 556 amino acid residue monomeric enzyme comprised of a catalytic domain that is most similar to the PKA, PKB, and PKC subfamily of protein kinases. |
| Accession # | NM_002613 |
| 运输条件 | Dry Ice |
| N-terminal Sequence Analysis | Using an N-terminal His tag |
| SDS-PAGE | 67 kDa |
| 存放说明 | -80℃ |
| 纯度 | >70%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 μg per lane. |
| 参考文献 |
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The approximate molecular weight is 67 kDa and the average purity is 85%. |
