| 货号 | 3804-KS-010 |
| 别名 | cell cycle regulated protein kinase; EC 2.7.11; EC 2.7.11.21; PLK; PLK-1; polo (Drosophia)-like kinase; polo like kinase; polo-like kinase 1polo-like kinase (Drosophila); Serine/threonine-protein kinase 13; serine/threonine-protein kinase PLK1; STPK13 | 全称 | Polo-like Kinase 1 |
| 反应种属 | Human |
| 来源 | Spodoptera frugiperda,Sf 9 (baculovirus)-derived Accession # NM_005030 |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 5347 (Human); 18817 (Mouse); 25515 (Rat) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. CIP2A modulates cell-cycle progression in human cancer cells by regulating the stability and activity of Plk1. | |
| 生物活性 | The activity of PLK1 is typically 14-20 nmol/min/mg using a dephospho-casein substrate (see Activity Assay Protocol). |
| 背景 | PLK1 is a member of the Polo-Like Kinase family that localizes to centrosomes or spindle pole bodies and undergoes dramatic subcellular relocation during the cell cycle. Deregulated activities of PLKs often result in abnormalities in centrosome duplication, maturation, and/or microtubule dynamics (1). PLKs also regulate the function of the Golgi complex. Deregulated expression of human PLK1 is strongly correlated with the development of many types of malignancies, and ectopic expression of PLK1 dominant negative protein leads to rapid cell death (2). |
| Accession # | NM_005030 |
| 运输条件 | Dry Ice |
| N-terminal Sequence Analysis | Using an N-terminal His tag |
| SDS-PAGE | 70 kDa |
| 存放说明 | -80℃ |
| 纯度 | >70%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 μg per lane |
| 参考文献 |
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The approximate molecular weight is 70 kDa and the average purity is 73%. |
