| 货号 | 3709-KS-010 |
| 别名 | 70 kDa zeta-associated protein; EC 2.7.10; EC 2.7.10.2; mrtle; mur; SRK; SRKFLJ17679; STD; STDFLJ17670; Syk-related tyrosine kinase; tyrosine-protein kinase ZAP-70; TZK; ZAP-70; zeta-chain (TCR) associated protein kinase (70 kD); zeta-chain (TCR) associated protein kinase 70kDa; zeta-chain associated protein kinase, 70kD | 全称 | Zeta-chain [TCR] Associated Protein Kinase 70 kDa |
| 反应种属 | Human |
| 来源 | Spodoptera frugiperda,Sf 9 (baculovirus)-derived Accession # NM_001079 |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 7535 (Human); 22637 (Mouse); 301348 (Rat) |
| 生物活性 | The activity of ZAP70 is typically 95-129 nmol/min/mg using a Poly (Glu:Tyr, 4:1) synthetic peptide substrate (see Activity Assay Protocol). |
| 背景 | ZAP70 is a non-receptor protein tyrosine kinase (part of the Syk/ZAP70 family) that is involved in signaling by the T-cell antigen receptor (TCR). Ligation of the TCR/CD3 receptor in Jurkat T-cells induces phosphoprotein complexes that contain ZAP70 (1). TCR zeta chains are initially phosphorylated by p56Lck that lead to the recruitment of ZAP70 via its SH2 domain. ZAP70 in turn phosphorylates other proteins in the TCR-phosphoprotein complex. One of the natural substrates for ZAP70 is the zeta-chain dimer of the TCR/CD3 complex (2). |
| Accession # | NM_001079 |
| 运输条件 | Dry Ice |
| N-terminal Sequence Analysis | Using an N-terminal GST tag |
| SDS-PAGE | 96 kDa |
| 存放说明 | -80℃ |
| 纯度 | >705%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 μg per lane |
| 参考文献 |
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The approximate molecular weight is 96 kDa and the average purity is 95%. |
