概述| 货号 | 3706-KS-010 |
| 别名 | EC 2.7.11; EC 2.7.11.1; HSPK 21; HsPK21; NEK2AHsPK21; Never in mitosis A-related kinase 2; NIMA (never in mitosis gene a)-related kinase 2; NimA-like protein kinase 1; NimA-related protein kinase 2; NLK1; NLK1serine/threonine-protein kinase Nek2 |
全称 | NIMA [Never In Mitosis Gene A]-related Kinase 2 | | 反应种属 | Human |
| 来源 | Spodoptera frugiperda,Sf 9 (baculovirus)-derived Accession # NM_002497 |
| 产品组分 | |
性能| 供应商 | R&D Systems |
| Entrez Gene IDs | 4751 (Human); 18005 (Mouse); 114482 (Rat) |
| 生物活性 | The activity of NEK2 is typically 237-321 nmol/min/mg using a myelin basic protein (MBP) substrate (see Activity Assay Protocol). |
| 背景 | NEK2 is closely related in its catalytic domain to the Serine/Threonine protein kinase NIMA of Aspergillus nidulans that is required for entry into mitosis and may function in parallel to the universal mitotic inducer p34cdc2. Like NIMA, the NEK2 protein is almost undetectable during G1 but accumulates progressively throughout S, reaching maximal levels in late G2 (1). NEK2 is shown to be expressed most abundantly in the testis of the adult tissues examined being localized to the nucleus (2). |
| Accession # | NM_002497 |
| 运输条件 | Dry Ice |
N-terminal
Sequence Analysis | Using an N-terminal GST tag |
| SDS-PAGE | 76 kDa |
| 存放说明 | -80℃ |
| 纯度 | >70%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 μg per lane |
| 参考文献 | - Schultz, S.J. et al. (1994) Cell Growth Differ. 5:625.
- Fry, A.M. et al. (1995) J. Biol. Chem. 270:12899.
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参考图片The approximate molecular weight is 76 kDa and the average purity is 90%. |
