| 货号 | 3389-KS-010 |
| 别名 | ASV; c-Src; EC 2.7.10; EC 2.7.10.2; pp60c-src; Rous sarcoma; RSVgp4; tyrosine kinase pp60c-src; tyrosine-protein kinase SRC-1; v-src avian sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog; v-src sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog (avian) | 全称 | v-src Sarcoma [Schmidt-Ruppin A-2] Viral Oncogene Homolog |
| 来源 | Spodoptera frugiperda,Sf 9 (baculovirus)-derived Accession # M11753 |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 6714 (Human); 20779 (Mouse); 83805 (Rat); 1491925 (Viral) |
| 生物活性 | The activity of Src is typically 99-133 nmol/min/mg using a synthetic peptide substrate (KVEKIGEGTYGVVYK) (see Activity Assay Protocol). |
| 背景 | The non-receptor tyrosine kinase Src was originally identified as a transforming protein of the Rous sarcoma virus that had enzymatic ability to phosphorylate tyrosine in protein substrates (1). The proto-oncogene c-Src is the cellular homologue of viral Src (v-Src) and the founding member of the Src family kinases. c-Src is over-expressed and activated in a large number of human maligancies and has been linked to the development of cancer and progression to distant metastases (2). In addition to increasing cell proliferation, a key role of c-Src in cancer seems to be the ability to promote invasion and motility, functions that might contribute to tumor progression. Although v-Src and c-Src share 88% amino acid identity, v-Src, unlike c-Src, is constitutively active mainly because it lacks a crucial c-terminal negative-regulatory region (3). As a result, v-Src is missing a tyrosine residue (Y530 in human c-Src) that upon phosphorylation contributes to c-Src assuming an inactive conformation. |
| Accession # | M11753 |
| 运输条件 | Dry Ice |
| N-terminal Sequence Analysis | Using an N-terminal GST tag |
| SDS-PAGE | 85 kDa |
| 存放说明 | -80℃ |
| 纯度 | >70%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 μg per lane |
| 参考文献 |
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The approximate molecular weight is 85 kDa and the average purity is 90%. |
