| 货号 | 1630-KS-010 |
| 别名 | Checkpoint, S. pombe, homolog of, 1; CHEK1; CHK1 (checkpoint, S.pombe) homolog; CHK1 checkpoint homolog (S. pombe); CHK1serine/threonine-protein kinase Chk1; EC 2.7.11; EC 2.7.11.1 | 全称 | Checkpoint Kinase 1 |
| 反应种属 | Human |
| 来源 | Spodoptera frugiperda,Sf 9 (baculovirus)-derived Accession # NM_001274 |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 1111 (Human); 12649 (Mouse); 140583 (Rat) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Checkpoint kinase 1-induced phosphorylation of O-linked ?-N-acetylglucosamine transferase regulates the intermediate filament network during cytokinesis | |
| 生物活性 | The activity of Chk1 is typically 169-229 nmol/min/mg using a synthetic peptide substrate (KKKVSRSGLYRSPSMPENLNRPR) (see Activity Assay Protocol). |
| 背景 | Chk1 is a 56 kDa Serine/Threonine protein kinase that was originally identified in fission yeast to play a role in activation of the DNA damage checkpoint in the G2 phase of the cell cycle (1). Chk1 appears to function downstream of several of the known fission yeast checkpoint gene products, including that encoded by Rad3, a gene with sequence similarity to the ATM gene mutated in patients with ataxia telangiectasia (2). |
| Accession # | NM_001274 |
| 运输条件 | Dry Ice |
| N-terminal Sequence Analysis | Using an N-terminal GST tag |
| SDS-PAGE | 82 kDa |
| 存放说明 | -80℃ |
| 纯度 | >70%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 μg per lane |
| 参考文献 |
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The approximate molecular weight is 82 kDa and the average purity is 95%. |
