| 货号 | 1358-KS-010 |
| 别名 | CDS1; CHEK2; CHK2 checkpoint homolog (S. pombe); EC 2.7.11; EC 2.7.11.1; HuCds1; LFS2; PP1425; Rad53; S.pombe) homolog | 全称 | Checkpoint Kinase 2 |
| 反应种属 | Human |
| 来源 | Spodoptera frugiperda,Sf 9 (baculovirus)-derived Accession # NM_007194 |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 11200 (Human); 50883 (Mouse); 114212 (Rat) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Chk2 phosphorylation of survivin-DeltaEx3 contributes to a DNA damage-sensing checkpoint in cancer. | |
| 生物活性 | The activity of Chk2 is typically 654-884 nmol/min/mg using a synthetic peptide substrate (KKKVSRSGLYRSPSMPENLNRPR) (see Activity Assay Protocol). |
| 背景 | Chk2 is rapidly phosphorylated and activated in response to replication blocks and DNA damage where the response to DNA damage occurs in an ataxia telangiectasia mutated (ATM)-dependent manner (1). Expression of wild-type Chk2 leads to increased p53 stabilization after DNA damage, whereas expression of a dominant-negative Chk2 mutant abrogates both phosphorylation of p53 on Serine 20 and p53 stabilization (2). |
| Accession # | NM_007194 |
| 运输条件 | Dry Ice |
| N-terminal Sequence Analysis | Using an N terminal GST tag |
| SDS-PAGE | 88 kDa |
| 存放说明 | -80℃ |
| 纯度 | >70%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 μg per lane |
| 参考文献 |
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The approximate molecular weight is 88 kDa and the average purity is 90%. |
