Recombinant Human PDGF-BB, Biotinylated Protein (10 UG)【科瑞奥博】

产品中心

当前位置：首页>产品中心

Recombinant Human PDGF-BB, Biotinylated Protein (10 UG)

货号： BT220-010 基本售价： 4451.6 元规格： 10 ug

产品信息

概述
货号	BT220-010
别名	PDGFBB; PDGF-BB
全称	Platelet-derived Growth Factor BB
反应种属	Human
来源	E. coli-derived Ser82-Thr190
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	5155 (Human); 24628 (Rat)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Silibinin Inhibits Platelet-Derived Growth Factor-Driven Cell Proliferation via Downregulation of N-Glycosylation in Human Tenon's Fibroblasts in a Proteasome-Dependent Manner PLoS ONE, 2016;11(12):e0168765. Species: Human Sample Type: Whole Cells Application: Bioassay
内毒素水平	<1.0 EU per 1 μg of the protein by the LAL method.
生物活性	Measured in a cell proliferation assay using NR6R‑3T3 mouse fibroblast cells. Raines, E.W. et al. (1985) Methods Enzymol. 109:749. The ED₅₀ for this effect is typically 1.5-6 ng/mL.
溶解方法	Reconstitute at 100 μg/mL in 4 mM HCl.
背景	Platelet-Derived Growth Factor (PDGF)-BB is synthesized as a 35 kDa, 241 amino acid (aa) prepro-precurser. It contains a signal peptide, an N-terminal prodomain, a mature region, and a C-terminal prodomain (1-4). The propercursor is initially dimerized and then intracellulary processed twice. The N-terminal prodomain is cleaved first, followed by cleavage of the C-terminal prodomain. The resulting mature region is 16-17 kDa in size (or 29-32 kDa as a homodimer) (4). Mature human PDGF-B shares 89% aa sequence identity with mouse mature PDGF-B. PDGF-BB is expressed by hepatocytes and nonresorbing osteoclasts, generating osteoblasts and bone formation (4, 5). It is also produced by platelets, macrophages, and mast cells. At sites of injury, it promotes neutrophil and macrophage infiltration for debridement, fibroblast secretion of new extracellular matrix, and IGF-I-mediated re-epithelialization (6, 7). The traditional receptor for PDGF is either a homodimer or heterodimer created from two type I transmembrane RTKs, PDGF R alpha and PDGF R beta (8, 9). PDGF-BB has been shown to bind the alpha alpha homodimer, alpha beta heterodimer, and the beta beta homodimer in vitro, and act through the beta beta homodimer in vivo (8, 10).
Accession #	Q6FHE7
运输条件	Blue Ice
预期分子量	12 kDa
SDS-PAGE	12 kDa, reducing conditions
存放说明	-20℃
纯度	>95%, by SDS-PAGE with silver staining.
不含载体	What does CF mean? CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA. What formulation is right for me? In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
参考文献	Rao, C.D. et al. (1986) Proc. Natl. Acad. Sci. USA 83:2392. Kaetzel, D.M. et al. (1996) Biochim. Biophys. Acta. 1298:250. Ostman, A. et al. (1992) J. Cell Biol. 118:509. Siegfried, G. et al. (2005) Oncogene 24:6925. Kreja, L. et al. (2010) J. Cell. Biochem. 109:347. van Steensel, L. et al. (2012) J. Clin. Endocrinol. Metab. 97:E400. Barrientos, S. et al. (2008) Wound Repair Regen. 16:585. Andrae, J. et al. (2008) Genes Dev. 22:1276. Heldin, C.H. and B. Westermark (1999) Physiol. Rev. 79:1283. Li, X. and U. Eriksson (2003) Cytokine Growth Factor Rev. 14:91.

参考图片

Both Biotinylated Recombinant Human PDGF-BB (Catalog # BT220) and unlabeled Recombinant Human PDGF-BB (Catalog # 220-BB) stimulate NR6R‑3T3 mouse fibroblast cell proliferation. The ED₅₀ for this effect is typically 1.5-6 ng/mL. The similarity in activity highlights that the biotinylated protein is fully functional.