| 货号 | BT496 | ||||||||
| 别名 | EFNB2; ELF-2; Htk-L; LERK-5; NLERK-1 | ||||||||
| 反应种属 | Mouse | ||||||||
| 来源 | Mouse myeloma cell line, NS0-derived
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| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 1948 (Human); 13642 (Mouse); 30219 (Zebrafish) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Structural basis of T-cell specificity and activation by the bacterial superantigen TSST-1. | |
| 内毒素水平 | <1.0 EU per 1 μg of the protein by the LAL method. |
| 生物活性 | Measured by its binding ability in a functional ELISA. Immobilized recombinant mouse EphB2 Fc Chimera at 2 µg/mL (100 µL/well) can bind Biotinylated Recombinant Mouse Ephrin-B2 Fc Chimera with a linear range of 0.039-2.5 ng/mL. Optimal dilutions should be determined by each laboratory for each application. |
| 溶解方法 | Reconstitute at 100 μg/mL in sterile PBS. |
| 背景 | Ephrin‑B2, also known as Htk‑L, ELF‑2, LERK‑5, and NLERK‑1, is a 40 kDa member of the Ephrin‑B family of transmembrane ligands that bind and induce the tyrosine autophosphorylation of Eph receptors. The extracellular domains of Ephrin‑B ligands are structurally related to GPI‑anchored Ephrin‑A ligands. Eph‑Ephrin interactions are widely involved in the regulation of cell migration, tissue morphogenesis, and cancer progression. Ephrin‑B2 preferentially interacts with receptors in the EphB family (1, 2). Mature mouse Ephrin‑B2 consists of a 204 amino acid (aa) extracelluar domain (ECD), a 21 aa transmembrane segment, and an 83 aa cytoplasmic domain [Cerretti 1197, Bergemann 4921, Bennett 1866]. Within the ECD, mouse Ephrin‑B2 shares 97% and 98% aa sequence identity with human and rat Ephrin‑B2, respectively. Ephrin‑B2 is expressed presynaptically on neurons (6, 7). It promotes presynaptic development, EphB2 shedding, axonal growth cone collapse, and neurite repulsion, and also regulates inflammatory and neuropathic pain (6 ‑ 8). Ephrin‑B2 is expressed by vascular mural cells and arterial vascular and lymphatic endothelium (9, 10). It exerts proliferative and migratory effects on these cells during angiogenesis and lymphangiogenesis in part by regulating the signaling activity of VEGF R2 and VEGF R3 (8 ‑ 11). Ephrin‑B2 plays a role in the immune response by mediating monocyte extravasation and T cell costimulation (12, 13). It is upregulated in invasive cancers and promotes tumor cell migration, invasion, and tumor angiogenesis (14 ‑ 16). It functions as a cellular entry receptor for Hendra and Nipah viruses (17). Ephrin‑B2 is also important for the separation of the urinary and intestinal tracts during development (18). |
| Accession # | AAA82934 |
| 运输条件 | Blue Ice |
| N-terminal Sequence Analysis | Arg27 |
| 预期分子量 | 49.6 (monomer) kDa |
| SDS-PAGE | 60-65 kDa, reducing conditions |
| 存放说明 | -20℃ |
| 纯度 | >90%, by SDS-PAGE under reducing conditions and visualized by silver stain |
| 参考文献 |
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