| 货号 | BT473 | ||||||||
| 别名 | Cek5-L; EFL-3; EFNB1; ELK-L; LERK-2; STRA-1 | ||||||||
| 反应种属 | Mouse | ||||||||
| 来源 | Mouse myeloma cell line, NS0-derived
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| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 1947 (Human); 13641 (Mouse); 25186 (Rat) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. EphrinB1 is essential in T-cell-T-cell co-operation during T-cell activation. | |
| 生物活性 | Measured by its binding ability in a functional ELISA. Immobilized recombinant mouse EphB3 Fc Chimera at 2 µg/mL (100 µL/well) can bind biotinylated Recombinant Mouse Ephrin‑B1 Fc Chimera with a linear range of 0.078-1.25 ng/mL. Optimal dilutions should be determined by each laboratory for each application. |
| 背景 | Ephrin-B1, also known as Elk Ligand, LERK2, and Eplg2, is an approximately 45 kDa member of the Ephrin-B family of transmembrane ligands that bind and induce the tyrosine autophosphorylation of Eph receptors. The extracellular domains (ECD) of Ephrin-B ligands are structurally related to GPI-anchored Ephrin-A ligands. Eph‑Ephrin interactions are widely involved in the regulation of cell migration, tissue morphogenesis, and cancer progression. Ephrin-B1 preferentially interacts with receptors in the EphB family. The binding of Ephrin-B1 to EphB proteins also triggers reverse signaling through Ephrin-B1 (1, 2). Mature mouse Ephrin-B1 consists of a 212 amino acid (aa) ECD, a 21 aa transmembrane segment, and an 88 aa cytoplasmic domain (3, 4). Within the ECD, mouse Ephrin-B1 shares 94% and 98% aa sequence identity with human and rat Ephrin-B1, respectively. Ligation by EphB2 enhances shedding of a 35 kDa fragment of the Ephrin-B1 ECD (5). The residual membrane-bound portion is then cleaved by gamma-secretase to release the intracellular domain (6). Ephrin-B1 also associates in cis with Claudin-1, -4, and -5 (7, 8). It is expressed on glomerular podocyte slit diaphragms, developing thymocytes, peripheral T cells, monocytes, macrophages, vascular endothelial cells, cardiomyocytes, osteoclasts, and luteinizing granulosa cells in the ovary (8-13). In the developing nervous system, Ephrin-B1 plays a role in cellular migration, axon guidance, and presynaptic development (14-16). It also regulates developing thymocyte survival, monocyte migration, osteoclast differentiation and function, cardiac muscle morphogenesis, and tumorigenesis (5, 8, 10-12). Ephrin-B1 is up‑regulated on reactive astrocytes and on macrophages and T cells found in atherosclerotic plaques (11, 17). |
| Accession # | Q544L9 |
| 运输条件 | Blue Ice |
| N-terminal Sequence Analysis | Lys30 |
| 预期分子量 | 49.2 (monomer) kDa |
| SDS-PAGE | 60 kDa, reducing conditions |
| 存放说明 | -20℃ |
| 纯度 | >95%, by SDS-PAGE under reducing conditions and visualized by silver stain |
| 参考文献 |
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