| 货号 | BT369 | ||||||||
| 别名 | EFL4; EFL-4; EFNA4; EPH-related receptor tyrosine kinase ligand 4; ephrin-A4; EPLG4MGC125826; LERK-4; LERK4FLJ57652; ligand of eph-related kinase 4 | ||||||||
| 反应种属 | Human | ||||||||
| 来源 | Mouse myeloma cell line, NS0-derived
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| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 1945 (Human); 13639 (Mouse) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Widespread potential for growth-factor-driven resistance to anticancer kinase inhibitors. | |
| 生物活性 | Measured by its binding ability in a functional ELISA. Immobilized rmEphA7/Fc Chimera at 2 µg/mL (100 µL/well) can bind biotinylated rhEphrin-A4/Fc Chimera with a linear range of 0.16-10 ng/mL. Optimal dilutions should be determined by each laboratory for each application. |
| 背景 | Ephrin-A4, also known as LERK-4 and EFL-4 (1), is a member of the ephrin ligand family which binds members of the Eph receptor family. All ligands share a conserved extracellular sequence, which most likely corresponds to the receptor binding domain. This conserved sequence consists of approximately 125 amino acids and includes four invariant cysteines. The A-class ligands have a GPI anchor following the conserved sequence. Ephrin-A4 has been shown to bind EphA2, EphA3, EphA4, EphA5, EphA6, EphA7, and EphB1 (2, 3). The extracellular domains of human and mouse ephrin-A4 share 80% amino acid identity. Only membrane-bound or Fc-clustered ligands are capable of activating the receptor in vitro. While soluble monomeric ligands bind the receptor, they do not induce receptor autophosphorylation and activation (2). In vivo, the ligands and receptors display reciprocal expression (3). It has been found that nearly all receptors and ligands are expressed in developing and adult neural tissue (3). The Eph/ephrin families also appear to play a role in angiogenesis (3). |
| Accession # | P52798 |
| 运输条件 | Blue Ice |
| N-terminal Sequence Analysis | Leu26 |
| 预期分子量 | 43.7 (monomer) kDa |
| SDS-PAGE | 50 kDa, reducing conditions |
| 存放说明 | -20℃ |
| 纯度 | >90%, by SDS-PAGE under reducing conditions and visualized by silver stain |
| 参考文献 |
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