Recombinant Mouse His6-UCH-L1 Protein, CF (25 UG)
货号：
E-335-025 基本售价：
1695.3 元 规格：
25 ug
产品信息
概述| 货号 | E-335-025 |
| 别名 | EC 3.4.19.12; Neuron cytoplasmic protein 9.5; PARK5; PGP 9.5; PGP9.5; PGP95; ubiquitin carboxyl-terminal esterase L1 (ubiquitin thiolesterase); ubiquitin carboxyl-terminal hydrolase isozyme L1; ubiquitin C-terminal hydrolase; Ubiquitin thioesterase L1; UCH-L1 |
全称 | Ubiquitin C-terminal Hydrolase L1 | | 反应种属 | Mouse |
| 来源 | E. coli-derived Contains a 6-His tag |
| 产品组分 | |
性能| 供应商 | R&D Systems |
| Entrez Gene IDs | 7345 (Human); 22223 (Mouse) |
| 生物活性 | Recombinant Mouse His6-UCH-L1 is a Ubiquitin-specific deconjugating enzyme. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Mouse His6-UCH-L1 concentration of 10-50 nM. A 15 minute pre-incubation with 10 mM DTT is recommended to achieve maximum activity. |
| 背景 | Ubiquitin Carboxyl-terminal Esterase L1 (UCH-L1), also known as PGP 9.5, is a deubiquitinating enzyme with a predicted molecular weight of 25 kDa (1). The human protein shares 95% amino acid sequence identity with its mouse and rat orthologs. UCH-L1 is expressed abundantly in neurons, accounting for 1-2% of total soluble proteins in the brain (2). It localizes primarily to the cytoplasm, but a subpopulation has been shown to be transiently nuclear (3,4). UCH-L1 contains two catalytic residues, Cys90 and His161, which are required for isopeptide bond cleavage at the C-terminal glycine residue of Ubiquitin (5). The levels of free Ubiquitin appear to be partially regulated by UCH-L1 through the hydrolysis of small Ubiquitin chains and the stabilization of monomeric Ubiquitin (6). Mice lacking functional UCH-L1 show neuronal dysfunction and neurodegeneration, and mutations in this enzyme have been linked to Parkinsons disease, suggesting that it is important for proper central nervous system function (7,8). UCH-L1 also likely plays a complex role in cancer. It has been reported to function as an oncogene in lymphoma, colorectal cancer, and nonsmall cell lung carcinoma (9-11). In contrast, it is thought to function as a tumor suppressor protein in nasopharyngeal and breast cancers (12,13). |
| Accession # | Q9R0P9 |
| 运输条件 | Dry Ice |
| 预期分子量 | 27 kDa |
| 存放说明 | -80℃ |
| 纯度 | >95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain |
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