货号 | MCA155EL |
克隆号 | OX-26 |
同种亚型 | IgG2a |
反应种属 | Rat |
来源宿主 | Mouse |
应用 | C, F, IF, P, WB |
供应商 | Bio-Rad Antibodies |
溶解方法 | Reconstitute with 1 ml distilled water |
运输条件 | |
存放说明 | Store at +4oC or at -20oC if preferred. This product should be stored undiluted. Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.Store at +4oC or at -20oC if preferred. This product should be stored undiluted. Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.Store at +4oC or at -20oC if preferred. This product should be stored undiluted. Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.Prior to reconstitution store at +4oC. Following reconstitution store at +4oC. DO NOT FREEZE. This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.Store at +4oC or at -20oC if preferred. This product should be stored undiluted. Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.Store at +4oC or at -20oC if preferred. This product should be stored undiluted. Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.Store at -20oC only. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. |
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Staining of mouse spleen cells with Mouse anti Rat CD71:Low Endotoxin (MCA155EL) | |
Staining of mouse spleen with Mouse anti Rat CD71:Biotin (MCA155B) | |
Staining of rat spleen cells with Mouse anti Rat CD71:RPE (MCA155PE) | |
Staining of rat spleen lymphocytes with Mouse anti Rat CD71 (MCA155G) | |
Published customer image: FITC conjugated Mouse anti Rat CD71 antibody, clone OX-26 used for the evaluation of CD71 expression on hair follicle stem cells by flow cytometry. Image caption: Flow cytometric analysis of follicle stem cells separated by Vario MACS. (a–d) Single-color flow cytometric analysis of the selected follicle stem cells. X axis represents fluorescence intensity, and Y axis represents cell number. (a) Bulge region cell suspension without antibody staining was used as control. (b) CD34bri cells were isolated by Vario MACS. (c) Control for α6 separation. (d) α6bri cells were isolated by Vario MACS. (e–h) Two-color flow cytometric analysis of follicle stem cells selected by Vario MACS. Cells were divided into four subpopulations: a PEbri FITCdim cells; b PEbri FITCbri cells; c PEdim FITCdim cells; and d PEdim FITCbri cells. (e) Control. (f) CD71 and CD34 was detected with FITC (X axis) and with PE (Y axis). The majority of the isolated cells were CD34briCD71dim cells, characterized by high level expression of CD34 and low level of CD71. No CD34dimCD71bri cells were detected in our experiments. (g) Control for α6 and CD71 combined separation; (h) The α6briCD71dim cells were the main subpopulation of the separated cells. From: Huang E, Lian X, Chen W, Yang T, Yang L. Characterization of rat hair follicle stem cells selected by vario magnetic activated cell sorting system. Acta Histochem Cytochem. 2009 Oct 30;42(5):129-36. |