货号 | FAB3250F |
别名 | CD146 antigen; CD146; cell surface glycoprotein MUC18; Cell surface glycoprotein P1H12; L-Gicerin; melanoma adhesion molecule; melanoma cell adhesion moleculeS-endo 1 endothelial-associated antigen; Melanoma-associated antigen A32; Melanoma-associated antigen MUC18; MUC18; MUC18Gicerin | 全称 | Melanoma Cell Adhesion Molecule |
反应种属 | Rat |
应用 | Flow Cytometry(10 µL/106cells) |
目标/特异性 | Detects rat MCAM/CD146 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) MCAM, rhBCAM, or rhALCAM is observed. |
使用方法 | Flow Cytometry: 10 µL/106cells |
来源 | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
产品组分 |
供应商 | R&D Systems |
Entrez Gene IDs | 4162 (Human); 84004 (Mouse); 78967 (Rat) |
应用文献 | |
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Sox10(+) adult stem cells contribute to biomaterial encapsulation and microvascularization | |
纯化方式 | Protein A or G purified from hybridoma culture supernatant |
免疫原 | Mouse myeloma cell line NS0-derived recombinant rat MCAM/CD146 Arg19-Lys560 Accession # Q9EPF2 |
生物活性 | Rat |
标记 | Fluorescein |
背景 | Melanoma Cell Adhesion Molecule (MCAM), also known as CD146 and MUC18, is a putative Ig-superfamily adhesion molecule that is expressed on endothelial cells and a variety of tumor cells. MCAM is associated with tumor progression and metastasis and may be involved in embryonic neural development. An alternately spliced isoform of rat MCAM has a 40 residue deletion in the cytoplasmic domain. Within the extracellular region, rat MCAM shares 72% and 90% amino acid sequence identity with human and mouse MCAM, respectively. |
运输条件 | Blue Ice |
存放说明 | 4℃ |
参考文献 |
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Detection of MCAM/CD146 in PC‑12 Rat Cell Line by Flow Cytometry. PC‑12 rat adrenal pheochromocytoma cell line was stained with Mouse Anti-Rat MCAM/CD146 Fluorescein‑conjugated Monoclonal Antibody (Catalog # FAB3250F, filled histogram) or isotype control antibody (Catalog # IC003F, open histogram). View our protocol for Staining Membrane-associated Proteins. |