| 货号 | AAR15G |
| 同种亚型 | Polyclonal IgG |
| 反应种属 | Rat |
| 来源宿主 | Rabbit |
| 应用 | E, FN, WB |
| 供应商 | Bio-Rad Antibodies |
| 溶解方法 | Reconstitute with 0.5ml sterile PBS containing 0.1% bovine serum albumin Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. |
| 运输条件 | |
| 存放说明 | Prior to reconstitution store at +4oC. Following reconstitution store at -20oC. This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.Prior to reconstitution store at +4oC. Following reconstitution store at -20oC. This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. |
| 本官网所有报价均为常温或者蓝冰运输价格，如有产品需要干冰运输，需另外加收干冰运输费。 |
Published customer image: Rabbit anti Rat Interleukin-1β antibody used for the detection of IL-1β expressing neurons in rat brain by immnofluorescence and immunohistochemistry on formalin fixed, paraffin embedded sections. Image caption: Neuronal expression of IL-1β in the right hemisphere exposed to lipopolysaccharide (LPS) + hypoxia-ischemia (HI). Mean ± standard error of the mean (SEM) (pg/mg) of IL-1β (A) and TNF-α (B). Titrations were done by ELISA on total protein extracts from the right cerebral hemisphere exposed to LPS plus HI for 4 h to 24 h compared to control (Ctl). (C) Immunofluorescent staining of neuronal nuclei (NeuN) + IL-1β + cells in the fronto-parietal neocortex (D). Mean ± SEM (%) of neural cells expressing Il-1β. (E) Immunohistochemical staining of IL-1β expression on neurons (arrows) from fronto-parietal neocortex and caudate putamen at 4 h post-LPS + HI compared to Ctl. Scale bar = 50 µm (4 to 6 pups per experimental condition). Analysis of variance with Newman-Keuls post-test; *P = 0.05, **P = 0.01. h hour, F-P Fronto-parietal, WM White matter, CPu Caudate putamen, Iba1 Ionized calcium-binding adapter molecule 1, GFAP Glial fibrillary acidic protein. From: Savard A, Lavoie K, Brochu ME, Grbic D, Lepage M, Gris D, Sebire G. Involvement of neuronal IL-1β in acquired brain lesions in a rat model of neonatal encephalopathy. J Neuroinflammation. 2013 Sep 5;10:110. |
