MOUSE ANTI RAT CD68:Alexa Fluor® 488【科瑞奥博】

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MOUSE ANTI RAT CD68:Alexa Fluor® 488

货号： MCA341A488 基本售价： 3882.0 元规格： 100 TESTS

产品信息

概述
货号	MCA341A488
克隆号	ED1
同种亚型	IgG1
反应种属	Rat
来源宿主	Mouse
应用	F*

性能
供应商	Bio-Rad Antibodies
溶解方法	Reconstitute with 1 ml distilled water
运输条件
存放说明	Prior to reconstitution store at +4^oC. Following reconstitution store at +4^oC. This product should be stored undiluted. DO NOT FREEZE. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.Store at +4^oC or at -20^oC if preferred. This product should be stored undiluted. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.Store at +4^oC or at -20^oC if preferred. This product should be stored undiluted. Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.Store at +4^oC or at -20^oC if preferred. This product should be stored undiluted. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.Store at +4^oC or at -20^oC if preferred. This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.Store at +4^oC or at -20^oC if preferred. This product should be stored undiluted. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.Store at +4^oC or at -20^oC if preferred. This product should be stored undiluted. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.Store at +4^oC or at -20^oC if preferred. This product should be stored undiluted. Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.

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参考图片

Staining of rat liver, with induced hepatocellular damage, with Mouse anti Rat CD68, clone ED1 (MCA341GA)

Staining of rat peritoneal macrophages withAlexa Fluor^®647 conjugated Mouse anti Rat CD68, clone ED1 (MCA341A647) following permeabilisation with Leucoperm (BUF09)

Staining of rat peritoneal macrophages with Alexa Fluor^® 488 conjugated Mouse anti Rat CD68, clone ED1 (MCA341A488). following permeabilisation with Leucoperm (BUF09)

Published customer image:
Mouse anti Rat CD68 antibody, clone ED1 used for the detection of macrophages in rat skin by immunohistochemistry.
Image caption:
Histological staining of control and HBOT wounds at post-wounding days 7 and 29. A–D) H&E staining. E–H) CD34 immunohistochemistry. I+J) CD68 immunohistochemistry.

From: uk B, Tong M, Fijneman EMG, van Neck JW (2014) Hyperbaric Oxygen Therapy to Treat Diabetes Impaired Wound Healing in Rats.
PLoS ONE 9(10): e108533.

Immunoperoxidase staining of rat lymph node cryosection with Mouse anti Rat CD68 antibody, clone ED1 (MCA341) followed by horseradish peroxidase conjugated Goat anti Mouse IgG1 (STAR132P) as a detection reagent. Low power

Published customer image:
Mouse anti Rat CD68 antibody, clone ED1 used for the detection of macrophages in rat liver by immunohistochemistry and western blotting.
Image caption:
Effects of RPE on Kupffer cells activation in liver. (a) Hepatic CD68 expression detected with immunohistology (original magnification, ×400), (b) Hepatic CD68 expression detected with western-blot. Values represent the mean ± SD of three independent experiments. *, versus control; #, versus ethanol.

From: Jing-Hua Peng, Tuan Cui, Zhao-Lin Sun, et al., “Effects of Puerariae Radix Extract on Endotoxin Receptors and TNF- Expression Induced by Gut-Derived Endotoxin in Chronic Alcoholic Liver Injury,”
Evidence-Based Complementary and Alternative Medicine, vol. 2012, Article ID 234987, 12 pages, 2012.

Published customer image:
Mouse anti Rat CD68 antibody, clone ED1 used for the detection of macrophages in rat brain by western blotting.
Image caption:
Western blotting of ED1 (Marker of activated microglia) and TRAIL (tumor necrosis factor-related apoptosis-inducing ligand). Brains were treated with PBS or rAAV2/IL12 and then implanted with tumor on the last day of Week 2 post viral vector injection. The brains were used for Western blotting analysis of the expression of ED1, 34 kDa, and TRAIL, 104 kDa, from microglial cells on the last day of week 1, 2, and 3 following tumor implantation. The brain slices for Western blotting analysis are shown at the bottom. All brains were harvested on the last day of week 1, week 2, and week 3 post tumor implantation. The scale bars indicate 2 mm in brain sections.

From: The treatment of glioblastoma multiforme through activation of microglia and TRAIL induced by rAAV2-mediated IL-12 in a syngeneic rat model. Chiu TL, Wang MJ, Su CC.
J Biomed Sci. 2012 Apr 22;19:45. doi: 10.1186/1423-0127-19-45.

Published customer image:
Mouse anti Rat CD68 antibody, clone ED1 used for the detection of macrophages in rat brain by immunohistochemistry.
Image caption:
Immunohistochemistry of ED1 stain in brain sections treated with rAAV2/IL12 (A, B, C, D, n = 4), AAV2/GFP (E, F, n = 2), or PBS (G, H, n = 2), accompanied with tumor implantation; and that in the brain section treated with nothing (n = 1). Immunohistochemistry of brain sections was performed for ED1 on the last day of week 3 after tumor implantation. The brain sections were stained with hematoxylin for nuclei and ED1 for activated microglial cells. The 1st column shows the brain sections pictured before immunostaining; the 2nd column shows the brain sections pictured after staining; the 3rd to 6th columns show pictures taken at four quadrants (black squares in 2nd column) of the tumor adjacent to the normal tissue in the right hemisphere. ED1-positive cells show dark brown. The scale bars indicate 2 mm in 1st and 2nd columns and 100 µm in 3rd-6th columns.

From: Chiu TL, Wang MJ, Su CC. The treatment of glioblastoma multiforme through activation of microglia and TRAIL induced by rAAV2-mediated IL-12 in a syngeneic rat model.
J Biomed Sci. 2012 Apr 22;19:45.

Published customer image:
Mouse anti Rat CD68 antibody, clone ED1 used for the detection of macrophages in rat brain by immunohistochemistry.
Image caption:
Immunohistochemical stains of ED1 and TRAIL in brain sections treated with AAV2/IL12 (A, B) or PBS (C, D), accompanied with tumor implantation; and that in the brain treated with nothing (E). Brain sections used in Figure 4 were also stained with hematoxylin for nuclei, ED1, and TRAIL, and pictured as in Figure 4. The 1st and 2nd columns show the low and high power fields of ED1 stain, respectively; the 3rd and 4th columns show the low and high power fields of TRAIL stain. Cells stained with ED1 or TRAIL show dark brown. The scale bars indicate 100 µm in low power field and 50 µm in high power field.

From: Chiu TL, Wang MJ, Su CC. The treatment of glioblastoma multiforme through activation of microglia and TRAIL induced by rAAV2-mediated IL-12 in a syngeneic rat model.
J Biomed Sci. 2012 Apr 22;19:45.

Immunofluorescence staining of rat lymph node cryosection with Mouse anti Rat CD68 antibody, clone ED1 (MCA341), red in A and Mouse anti Rat CD4 (MCA55), green in B. C is the merged image with nuclei counter-stained blue using DAPI. Low power

Published customer image:
Mouse anti Rat CD68 antibody, clone ED1 used for the detection of macrophages in rat brain by flow cytometry.
Image caption:
Fluorescence-activated cell sorting analysis of cells isolated from adjuvant-induced arthritis rat synovial tissue. Twenty-four hours after systemic administration of N-(2-hydroxypropyl)methacrylamide copolymer labeled with Alexa Fluor® 488 (P-Alexa), cells were isolated from adjuvant-induced arthritis rat synovial tissue for fluorescence-activated cell sorting (FACS) analysis. IgG1 was used as control for CD68 and prolyl-4-hydroxylase. IgG2a was used as control for CD11c. The result for IgG2a is similar to that for IgG1 with the upper right quadrant detected at 2.45% (data not shown).

From:Quan et al. Arthritis Research & Therapy 2010 12:R170.