| 货号 | MCA773PE |
| 克隆号 | 1A29 |
| 同种亚型 | IgG1 |
| 反应种属 | Rat |
| 来源宿主 | Mouse |
| 应用 | F |
| 供应商 | Bio-Rad Antibodies |
| 溶解方法 | Reconstitute with 1 ml distilled water |
| 运输条件 | |
| 存放说明 | Store at +4oC or at -20oC if preferred. This product should be stored undiluted. Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.Store at +4oC or at -20oC if preferred. This product should be stored undiluted. Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.Store at +4oC or at -20oC if preferred. This product should be stored undiluted. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.Store at -20oC only. This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.Pack Size: 0.2 mgStore at +4oC or at -20oC if preferred. This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.Pack Size: 0.1 mgStore at +4oC or at -20oC if preferred. This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.Store at +4oC or at -20oC if preferred. This product should be stored undiluted. Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.Prior to reconstitution store at +4oC. Following reconstitution store at +4oC. DO NOT FREEZE. This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.Store at +4oC or at -20oC if preferred. This product should be stored undiluted. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.Pack Size: 0.2 mgStore at +4oC or at -20oC if preferred. This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.Pack Size: 0.1 mgStore at +4oC or at -20oC if preferred. This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. |
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Published customer image: Mouse anti Rat CD54 antiody, clone 1A29 used for the evaluation of ICAM-1 expression in rat corneal grafts by immunofluorescence. Image caption: Immunohistochemistry staining for CD4+, CD8+, CD11c+, CD163+, CD54+, and CD25+ cells at central grafts for different groups. The syngeneic grafts were nearly free of immunologic cell infiltration. The grafts treated with the PA microfilms or PA eye drops had less dense and diffuse cell infiltration in comparison with the allogeneic control grafts. G: Graft. H: Host. Original magnification ×100. Scale bar: 100 μm. From: Liu Y-C, Peng Y, Lwin NC, Venkatraman SS, Wong TT, Mehta JS (2013) A Biodegradable, Sustained-Released, Prednisolone Acetate Microfilm Drug Delivery System Effectively Prolongs Corneal Allograft Survival in the Rat Keratoplasty Model. PLoS ONE 8(8): e70419. | |
Published customer image: Mouse anti Rat CD54 antiody, clone 1A29 used for the evaluation of ICAM-1 expression in rat corneal grafts by immunofluorescence. Image caption: Immunohistochemistry staining for CD4+, CD8+, CD11c+, CD163+, CD54+, and CD25+ cells at graft-host junction for different groups. The grafts treated with the PA microfilms or PA eye drops had less dense and diffuse cell infiltration in comparison with the allogeneic control grafts. The syngeneic grafts were nearly free of immunologic cell infiltration. G: Graft. H: Host. Original magnification ×100. Scale bar: 100 μm. From: Liu Y-C, Peng Y, Lwin NC, Venkatraman SS, Wong TT, Mehta JS (2013) A Biodegradable, Sustained-Released, Prednisolone Acetate Microfilm Drug Delivery System Effectively Prolongs Corneal Allograft Survival in the Rat Keratoplasty Model. PLoS ONE 8(8): e70419. | |
Published customer image: Mouse anti Rat CD54 antibody, clone 1A29 used for the evaluation of ICAM-1 expression in rat brain by immunohistochemistry on formalin fixed cryosections along with activated microglial presence using Mouse anti Rat CD11b antibody, clone OX-42 (MCA275R). Image caption: Evaluation of the effects of the PPAR-α agonist fenofibrate administered during the acute phase of experimental stoke in combination with thrombolysis with tPA on neuronal and vascular compartments. Evaluation takes place 72?h after the induction of middle cerebral artery occlusion in the rat. Fenofibrate (50?mg/kg/day) or vehicle was administered by twice-daily gavage over 72?h. Values are means ± SEM. (d) Expression of ICAM-1 adhesion protein, a marker of leukocyte/endothelium interactions, was increased during ischemia and thrombolysis but was lowered by fenofibrate treatment ( per group; ); scale bar, 100?μm. f) Expression of CD11b by Ox 42 antibody, a marker of microglial activation, was increased during ischemia and thrombolysis but was lowered by fenofibrate treatment ( per group; ); scale bar, 25?μm. From: Gautier, S et al. PPAR-Alpha Agonist Used at the Acute Phase of Experimental Ischemic Stroke Reduces Occurrence of Thrombolysis-Induced Hemorrhage in Rats. PPAR Research, vol. 2015, Article ID 246329, 6 pages, 2015. | |
Published customer image: Mouse anti Rat CD54 antibody, clone 1A29 used for the evaluation of ICAM-1 expression in brain tissue by western blotting in a focal cerebral ischemia model. Image caption: Western blot analysis of phosphorylated ERK1/2 and ICAM-1. (a) Phosphorylation of ERK1/2 was significantly higher in hypothermic (33) brains than normothermic (37) ones at 15, 30, and 120 minutes after ischemic insult (n = 6 per group). Total ERK1/2 level was not changed. (b) Ischemia-induced increase of ICAM-1 at 24 hours after MCAO was suppressed by hypothermia. But this suppression was not observed when ERK1/2 inhibition was done (U0126, n = 4) at the dose which almost completely inhibited ERK1/2 phosphorylation. *P < .05 versus normothermia. From: Choi JS, Park J, Suk K, Moon C, Park YK, Han HS. Mild Hypothermia Attenuates Intercellular Adhesion Molecule-1 Induction via Activation of Extracellular Signal-Regulated Kinase-1/2 in a Focal Cerebral Ischemia Model. Stroke Res Treat. 2011;2011:846716. | |
Published customer image: Mouse anti RatCD54 antibody, clone 1A29 used for the evaluation of ICAM-1 expression in rat corneal tissue by immunofluorescence. Image caption: Double immunostaining with anti-CD54 ICAM-1 (in green) and with propidium iodide (in red) on corneas. The corneas were analyzed with a confocal microscope with a 200 X enlargement. Immunofluorescence of ICAM-1 is very weak in control animals (A) and was increased 24 h after the LPS injection (B). Some polymorphonuclear cells (arrow) are visible on the endothelium. Double immunofluorescence with IgG1 mouse antibody for isotypic control (revealed by secondary Alexa Fluor in green) and with propidium iodide (in red) was performed in EIU (C) with a 250X enlargement. Intensities of staining were the same for each assay. From: Trinh L, Brignole-Baudouin F, Labbé A, Raphaël M, Bourges JL, Baudouin C. The corneal endothelium in an endotoxin-induced uveitis model: correlation between in vivo confocal microscopy and immunohistochemistry. Mol Vis. 2008 Jun 16;14:1149-56. | |
Published customer image: Mouse anti Rat CD54 antibody, clone 1A29 used for the evaluation of CD54 expression on rat mesenteric venules by immunohistochemistry on formalin fixed, paraffin embedded tissue sections. Image caption: In vivo leukocyte trafficking in rat mesenteric post-capillary venules. Animals were i.p. injected with 10 mL of PBS alone (C) or supplemented with D-glucose (DG; 40 mg/kg), either in the absence or presence of IL-1β (200 ng/kg). L-glucose (LG; 40 mg/kg) was used as an osmotic control. After 18 h, (A) leukocyte rolling flux, (B) leukocyte rolling velocity, (C) leukocyte adhesion and (D) leukocyte migration were determined by intravital microscopy. *P<0.05 versus matched group without IL-1β †P<0.05 versus IL-1β in PBS. Panel E shows representative photomicrographs showing ICAM-1 and VCAM-1 immunolocalization and leukocyte adhesion and transmigration in post-capillary venules. The brown reaction product indicates positive staining. Adhered and transmigrated leukocytes are marked with arrows in the right column. All panels are lightly counterstained with hematoxylin (x400). From: Azcutia V, Abu-Taha M, Romacho T, Vázquez-Bella M, Matesanz N, Luscinskas FW, et al. (2010) Inflammation Determines the Pro-Adhesive Properties of High Extracellular D-Glucose in Human Endothelial Cells In Vitro and Rat Microvessels In Vivo. PLoS ONE 5(4): e10091. | |
Staining of stimulated rat spleen cells with Mouse anti Rat CD54:FITC (MCA773F) | |
Staining of stimulated rat spleen cells with Mouse anti Rat CD54:Alexa Fluor® 488 (MCA773A488) | |
Staining of frozen rat spleen with Mouse anti Rat CD54 (MCA773) |
