MOUSE ANTI RAT CD8 ALPHA【科瑞奥博】

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MOUSE ANTI RAT CD8 ALPHA

货号： MCA48R 基本售价： 2931.0 元规格： 0.25 mg

产品信息

概述
货号	MCA48R
克隆号	OX-8
同种亚型	IgG1
反应种属	Rat
来源宿主	Mouse
应用	C, F, IF, IP, P, WB

性能
供应商	Bio-Rad Antibodies
溶解方法	Reconstitute with 1.0 ml distilled waterReconstitute with 1 ml distilled water
运输条件
存放说明	Store at +4^oC. DO NOT FREEZE. This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.Pack Size: 0.1 mg, 1 mgStore at +4^oC or at -20^oC if preferred. This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.Pack Size: 0.25 mgStore at +4^oC or at -20^oC if preferred. This product should be stored undiluted. Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.Store at +4^oC or at -20^oC if preferred. This product should be stored undiluted. Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.Pack Size: 0.1 mg, 1 mgStore at +4^oC or at -20^oC if preferred. This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.Pack Size: 0.25 mgStore at +4^oC or at -20^oC if preferred. This product should be stored undiluted. Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.Prior to reconstitution store at +4^oC. Following reconstitution store at +4^oC. DO NOT FREEZE. This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.Store at +4^oC or at -20^oC if preferred. This product should be stored undiluted. Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.Store at +4^oC or at -20^oC if preferred. This product should be stored undiluted. Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.Store at +4^oC or at -20^oC if preferred. This product should be stored undiluted. Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.Store at +4^oC or at -20^oC if preferred. This product should be stored undiluted. Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.Store at -20^oC only. This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.Pack Size: 0.1 mg, 1 mgStore at +4^oC or at -20^oC if preferred. This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.Pack Size: 0.25 mgStore at +4^oC or at -20^oC if preferred. This product should be stored undiluted. Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.

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参考图片

Staining of rat peripheral blood cells with Mouse anti Rat CD8 Alpha Chain:Alexa Fluor^® 488 (MCA48A488)

Published customer image:
Mouse anti Rat CD8 alpha antibody, clone OX-8 used for the identification of cytotoxic T cells in tumors by immunofluorescence.
Image caption:
Immunohistochemical labeling of tumor thin sections with CD8 (red) antibodies and DAPI (blue) nuclear stain. A: Two typical sections from an untreated control tumor collected 14 days after injecting tumor cells into the liver; B. Two typical sections of a secondary tumor that was fixed 7 days after it was injected into a liver in which the primary tumor had been nanoelectroablated 3 weeks earlier. This secondary tumor exhibited growth inhibition and a photograph of it can be seen in Fig 5B; C. Percentage of cells expressing CD8 in histological sections of tumors from four different conditions. Each bar represents the mean of measurements taken from 3–7 sections of 2 separate tumors. The untreated tumors were fixed 14 days after injection; “ns+7d” represents tumors fixed 7days after treating with 400 pulses, 15 kV, 100ns; “T2+7d” represents secondary tumors fixed 7 days after injection. CD8 concentration is significantly different from control tumor levels with **p = 0.002; “T2+7d+CD9 AB” represents secondary tumors fixed 7 days after injection with CD8 antibodies injected IP 24 h prior to the tumor cell injection. The CD8 concentration is significantly different from control tumors with *p = 0.005.

From:Citation: Nuccitelli R, Berridge JC, Mallon Z, Kreis M, Athos B, Nuccitelli P (2015) Nanoelectroablation of Murine Tumors Triggers a CD8-Dependent Inhibition of Secondary Tumor Growth.
PLoS ONE 10(7): e0134364.

Published customer image:
Mouse anti Rat CD8α antibody, clone OX-8 used for the detection of CD8 positive cells by flow cytometry.
Image caption
Qualitative and quantitative flow cytometric analysis of lymphocyte populations in draining lymph nodes. A: Representative FACS plot of CD4+ CD8+ staining used to count T-helper cells, cytotoxic T-cells and CD4+ CD8+ double positive T-lymphocytes. Events acquired: 2×105. B: FACS plot example for B-cell detection. C: Representative FACS plot for NK cell assessment. NK-T cell were confirmed by CD3 expression (not shown). Bar diagrams: Cumulative results for the quantification of major and minor lymphocyte populations in draining LN of cornea transplanted animals. An asterisk (*) indicates statistical significance at p=0.05 determined by Mann–Whitney U-Test. Allo-Tx-d7 - animals allo-grafted and analyzed at day 07 post op, n=6; allo-Tx-rej – animals displaying allo rejection of grafted corneas analyzed after the onset of rejection, n=5; syn-Tx-d7 – syngeneically grafted animals analyzed at day 7 post-op, n=3; syn-Tx-LT – syn-grafted long-term survivors analyzed at the end of the observation period at day 42; n=3.

Maenz M, Morcos M, Ritter T. A comprehensive flow-cytometric analysis of graft infiltrating lymphocytes, draining lymph nodes and serum during the rejection phase in a fully allogeneic rat cornea transplant model. Mol Vis. 2011 Feb 8;17:420-9.

Published customer image:
Mouse anti Rat CD8α antibody, clone OX-8 used for the detection of CD8 positive cells by immunohistochemistry.
Image caption
CD4 and CD8 T cell infiltration. Photos show SN sections of an animal of the cell death group immunostained with antibody against CD4 (A and C) and CD8 (B and D). The small panels show insets in A (C) and in B (D) at higher magnification. Scale: 50 µm, applies to A–B, 10 µm applies to C–D. (E) Graph shows average (dash) and individual numbers of CD4+ cells found in one SN section per animal of each group plotted per time. Two-way ANOVA [F (8,42) = 4.1, p = 0.001 effect of group and time interaction] followed by Tukey HSD post-hoc analysis. ## or ç p<0.01 (##) compared to the other a-syn group at same time point; (††) different to the next time point of the same group. (F) Graph shows average (dash) and individual numbers of CD8+ cells found in one SN section per animal of each group plotted per time. Two-way ANOVA [F (8,41) = 4.3, p = 0.001 effect of group and time interaction] followed by Tukey HSD post-hoc analysis. (**) p<0.01 compared to all other groups at all time point.

From: Sanchez-Guajardo V, Febbraro F, Kirik D, Romero-Ramos M (2010) Microglia Acquire Distinct Activation Profiles Depending on the Degree of α-Synuclein Neuropathology in a rAAV Based Model of Parkinson's Disease.
PLoS ONE 5(1): e8784.

Published customer image:
Mouse anti Rat CD8α antibody, clone OX-8 used for the detection of CD8 positive cells by immunohistochemistry.
Image caption
Analysis of graft-infiltrating T cells. Activated CD25+ T cells and CD4+ and CD8+ T cell subsets were stained at the time points of corneal allograft rejection and calculated within the graft. A, C, and E show representative histological staining for CD25, CD4, and CD8 in grafts of treated and control animals, respectively. CD25+ (B) and CD4+ (D) cells infiltrated to a statistically significantly stronger extent in 3.2.3-treated animals when compared to control treated animals (*p<0.01, ** p<0.01). No statistical difference was observed for CD8+ T cells (NS=not significant; F).

From: Schwartzkopff J, Schlereth SL, Berger M, Bredow L, Birnbaum F, Böhringer D, Reinhard T. NK cell depletion delays corneal allograft rejection in baby rats. Mol Vis. 2010 Oct 2;16:1928-35.

Published customer image:
Mouse anti Rat CD8α antibody, clone OX-8 used for the detection of CD8 expressing cells in Bouin's fixed, paraffin embedded tissue sections by immunofluorescence.
Image caption:
CD3ε, CD8α, and PAX5 immunostainings. (A–D) CD3ε staining in the zone of the foramen of Monro (fM) of the injected ventricle comprising a part of the stria medullaris [sm, (A)]. Many positive cells occurred in the perivascular spaces of the local large venules 12 h after NA injection. A detailed view (B) of the vessels in the stria medullaris (sm) shows that many positive cells have smooth nuclei while polymorphonucleated cells (arrow) are negative. (C) Twenty-four hours after the injection, some positive cells seemed to cross the ependyma of the choroid plexus (cp). (D) Positive cells were also present in the meninges (men) next to the optic chiasm (oc), and few appeared to penetrate the brain parenchyma (arrow). (E–G) Double immunofluorescence of lymphocytes in a 12 h post-injection brain section. Most CD3ε-positive cells [(E), green] found in the perivascular spaces of the large vessels were also positive for CD8α [(F), red]. (H–J) PAX5 immunostaining showing positive cells in the wall of the large vessels around the foramen of Monro [fM; (H)], although they were not as abundant as CD3ε-positive cells. Few PAX5-positive nuclei were also found in the choroid plexus (cp) of the injected lateral ventricle (I) as well as in the meninges (men) close to the optic chiasm [oc; (J)]. cp, choroid plexus; fM, foramen of Monro; men, meninges; oc, optic chiasm; sm, stria medullaris.

From: Granados-Durán P, López-Ávalos MD, Grondona JM, Gómez-Roldán Mdel C, Cifuentes M, Pérez-Martín M, Alvarez M, Rodríguez de Fonseca F, Fernández-Llebrez P. Neuroinflammation induced by intracerebroventricular injection of microbial neuraminidase. Front Med (Lausanne). 2015 Mar 17;2:14.

Immunofluorescence staining of rat lymph node cryosection with Mouse anti Rat CD25 antibody, clone OX-35 (MCA153), red in A and Mouse anti Rat CD8, clone OX-8 (MCA48), green in B. C is the merged image with nuclei counterstained blue using DAPI. High power

Immunofluorescence staining of rat lymph node cryosection with Mouse anti Rat CD25 antibody, clone OX-35 (MCA153), red in A and Mouse anti Rat CD8 antibody, clone OX-8 (MCA48), green in B. C is the merged image with nuclei counterstained blue using DAPI. Low power

Immunofluorescence staining of rat lymph node cryosection with Mouse anti Rat CD11b antibody, clone OX-42 (MCA275), red in A and Mouse anti Rat CD8 antibody, clone OX-8 (MCA48), green in B. C is the merged image with nuclei counter-stained in blue using DAPI. High power

Immunofluorescence staining of rat lymph node cryosection with Mouse anti Rat CD163 antibody, clone ED2 (MCA342), red an A and Mouse anti Rat CD8 antibody, clone OX-8 (MCA48), green in B. C is the merged image with nuclei counter-stained blue using DAPI. High power