| 货号 | FAB5831P |
| 别名 | BB2; CD54 antigen; CD54; cell surface glycoprotein P3.58; human rhinovirus receptor; ICAM-1; intercellular adhesion molecule 1 (CD54), human rhinovirus receptor; intercellular adhesion molecule 1; Major group rhinovirus receptor; P3.58 | 全称 | Intercellular Adhesion Molecule 1 |
| 反应种属 | Rat |
| 应用 | Flow Cytometry |
| 目标/特异性 | Detects rat ICAM‑1/CD54 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant mouse (rm) DCC, recombinant human (rh) ICAM-1, rmICAM-1, rmICAM-2, rhICAM-3, rmICAM-5, rmMAdCAM-1, rhCD31 or rmVCAM-1 is observed. |
| 使用方法 | Flow Cytometry: 10 µL/106cells |
| 来源 | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 3383 (Human); 15894 (Mouse); 25464 (Rat) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Epigenetic regulation of tumor endothelial cell anergy: silencing of intercellular adhesion molecule-1 by histone modifications. | |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant rat ICAM‑1/CD54 Gln28-Thr493 (predicted) Accession # Q00238 |
| 生物活性 | Rat |
| 标记 | Phycoerythrin |
| 背景 | Intercellular Adhesion Molecule-1 (ICAM-1, CD54), binds the leukocyte integrins LFA-1 ( alpha L/ beta 2 or CD11a/CD18) and Mac-1 ( alpha M/ beta 2 or CD11b/CD18). ICAM-1 expression is weak on leukocytes, epithelial and resting endothelial cells, as well as some other cell types, but expression can be stimulated by IFN-gamma, TNF-alpha, IL-1 beta, and LPS. Within the extracellular domain, rat ICAM-1 shares 53% and 78% amino acid sequence identity with human and mouse ICAM-1, respectively. Soluble ICAM-1 is found in a biologically active form in serum, probably as a result of proteolytic cleavage from the cell surface, and is elevated in patients with various inflammatory syndromes such as septic shock, leukocyte adhesion deficiency syndrome (LAD), cancer, and transplantation. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of ICAM‑1/CD54 in Rat Splenocytes by Flow Cytometry. Rat splenocytes were stained with Mouse Anti-Rat ICAM‑1/CD54 PE‑conjugated Monoclonal Antibody (Catalog # FAB5831P, filled histogram) or isotype control antibody (Catalog # IC0041P, open histogram). View our protocol for Staining Membrane-associated Proteins. |
